Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1783083..1783585 Replicon chromosome
Accession NZ_CP085637
Organism Escherichia coli strain fECg99.1

Toxin (Protein)


Gene name yoeB Uniprot ID D8A584
Locus tag LJU33_RS08475 Protein ID WP_000767828.1
Coordinates 1783331..1783585 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag LJU33_RS08470 Protein ID WP_001259255.1
Coordinates 1783083..1783334 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU33_RS08445 (1778261) 1778261..1779328 - 1068 WP_000080063.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
LJU33_RS08450 (1779328) 1779328..1780398 - 1071 WP_000108915.1 histidinol-phosphate transaminase -
LJU33_RS08455 (1780395) 1780395..1781699 - 1305 WP_001367820.1 histidinol dehydrogenase -
LJU33_RS08460 (1781705) 1781705..1782604 - 900 WP_000131783.1 ATP phosphoribosyltransferase -
LJU33_RS08465 (1782750) 1782750..1782800 - 51 WP_001364200.1 his operon leader peptide -
LJU33_RS08470 (1783083) 1783083..1783334 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
LJU33_RS08475 (1783331) 1783331..1783585 + 255 WP_000767828.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
LJU33_RS08480 (1783668) 1783668..1784492 + 825 WP_000754758.1 SDR family oxidoreductase -
LJU33_RS08485 (1784538) 1784538..1785467 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
LJU33_RS08490 (1785682) 1785682..1785744 + 63 WP_010723108.1 membrane protein YoeI -
LJU33_RS08495 (1785734) 1785734..1787092 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.56 Da        Isoelectric Point: 8.0353

>T221006 WP_000767828.1 NZ_CP085637:1783331-1783585 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T221006 NZ_CP117327:c4079441-4079157 [Salmonella enterica subsp. enterica serovar Saintpaul]
ATGACTTATGAACTGGAAATCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT221006 WP_001259255.1 NZ_CP085637:1783083-1783334 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT221006 NZ_CP117327:c4079673-4079431 [Salmonella enterica subsp. enterica serovar Saintpaul]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTTTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G465

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References