Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 591812..592611 Replicon chromosome
Accession NZ_CP085637
Organism Escherichia coli strain fECg99.1

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag LJU33_RS02920 Protein ID WP_000347273.1
Coordinates 591812..592276 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LJU33_RS02925 Protein ID WP_001307405.1
Coordinates 592276..592611 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU33_RS02890 (586813) 586813..587247 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LJU33_RS02895 (587265) 587265..588143 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LJU33_RS02900 (588133) 588133..588912 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LJU33_RS02905 (588923) 588923..589396 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LJU33_RS02910 (589419) 589419..590699 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
LJU33_RS02915 (590948) 590948..591757 + 810 WP_000072180.1 aga operon transcriptional regulator AgaR -
LJU33_RS02920 (591812) 591812..592276 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LJU33_RS02925 (592276) 592276..592611 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LJU33_RS02930 (592760) 592760..594331 - 1572 WP_001273741.1 galactarate dehydratase -
LJU33_RS02935 (594706) 594706..596040 + 1335 WP_023156343.1 galactarate/glucarate/glycerate transporter GarP -
LJU33_RS02940 (596056) 596056..596826 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T221002 WP_000347273.1 NZ_CP085637:c592276-591812 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T221002 NZ_CP117327:2329613-2329897 [Salmonella enterica subsp. enterica serovar Saintpaul]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT221002 WP_001307405.1 NZ_CP085637:c592611-592276 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT221002 NZ_CP117327:2329375-2329623 [Salmonella enterica subsp. enterica serovar Saintpaul]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References