Detailed information of TA system TA220989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1636688..1637348
• Replicon: chromosome
• Accession: NZ_CP085633
• Organism: Pectobacterium brasiliense strain TS20HJ1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LJQ72_RS07200
• Protein ID: WP_205567947.1
• Coordinates: 1636688..1637041 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJQ72_RS07205
• Protein ID: WP_010284412.1
• Coordinates: 1637046..1637348 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJQ72_RS07175 (LJQ72_07175)	1632012..1632674	-	663	WP_080163777.1	DUF3313 domain-containing protein	-
LJQ72_RS07180 (LJQ72_07180)	1633217..1634188	+	972	WP_039468449.1	chemotaxis protein	-
LJQ72_RS07185 (LJQ72_07185)	1634304..1635143	-	840	WP_010284408.1	glutathione-dependent disulfide-bond oxidoreductase	-
LJQ72_RS07190 (LJQ72_07190)	1635308..1635919	-	612	WP_039287426.1	NAD(P)-dependent oxidoreductase	-
LJQ72_RS07195 (LJQ72_07195)	1636022..1636462	+	441	WP_227527979.1	helix-turn-helix domain-containing protein	-
LJQ72_RS07200 (LJQ72_07200)	1636688..1637041	+	354	WP_205567947.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LJQ72_RS07205 (LJQ72_07205)	1637046..1637348	+	303	WP_010284412.1	helix-turn-helix transcriptional regulator	Antitoxin
LJQ72_RS07210 (LJQ72_07210)	1637386..1638390	-	1005	WP_227527980.1	threonine/serine dehydratase	-
LJQ72_RS07215 (LJQ72_07215)	1638443..1638793	+	351	WP_014914858.1	metalloregulator ArsR/SmtB family transcription factor	-
LJQ72_RS07220 (LJQ72_07220)	1638805..1640064	-	1260	WP_227527981.1	MFS transporter	-
LJQ72_RS07225 (LJQ72_07225)	1640131..1640586	+	456	WP_039287413.1	helix-turn-helix domain-containing protein	-
LJQ72_RS07230 (LJQ72_07230)	1640790..1642226	+	1437	WP_227527982.1	6-phospho-beta-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13530.57 Da
• Isoelectric Point: 10.0708

>T220989 WP_205567947.1 NZ_CP085633:1636688-1637041 [Pectobacterium brasiliense]
VWKIETTDVFDSWFSTLNDRDRAYVLAALIVLREKGPMLSRPYADTVKGSRYSNMKELRIQSGGMPIRAFFAFDPHRTGI
LLCAGNKSGNEKRFYDEMIPLADREFTKYLKKSEKRG

Nucleotide

• Length: 354 bp

>T220989 NZ_CP117326:3421477-3421695 [Salmonella enterica subsp. enterica serovar Newport]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11107.90 Da
• Isoelectric Point: 6.2283

>AT220989 WP_010284412.1 NZ_CP085633:1637046-1637348 [Pectobacterium brasiliense]
MGRTLEQLLADEKPDVVVEAHAIAADMLLNIHLAELRERVQKTQVEMAQVLGLKQPTIAGMEKPGRDLKLSTLKRYVEAT
GSKLRLDVELPDGSHYGFTL

Nucleotide

• Length: 303 bp

>AT220989 NZ_CP117326:3421075-3421449 [Salmonella enterica subsp. enterica serovar Newport]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA