Detailed information of TA system TA220978

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 23474..24146
• Replicon: chromosome
• Accession: NZ_CP085633
• Organism: Pectobacterium brasiliense strain TS20HJ1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LJQ72_RS00115
• Protein ID: WP_039511562.1
• Coordinates: 23760..24146 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A433N287
• Locus tag: LJQ72_RS00110
• Protein ID: WP_010281167.1
• Coordinates: 23474..23773 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJQ72_RS00090 (LJQ72_00090)	18745..19716	+	972	WP_227527508.1	nucleoside hydrolase	-
LJQ72_RS00095 (LJQ72_00095)	19716..20693	+	978	WP_039317548.1	ABC transporter permease	-
LJQ72_RS00100 (LJQ72_00100)	20690..21598	+	909	WP_039275990.1	ABC transporter permease	-
LJQ72_RS00105 (LJQ72_00105)	21638..23437	+	1800	WP_227527509.1	ABC transporter ATP-binding protein	-
LJQ72_RS00110 (LJQ72_00110)	23474..23773	-	300	WP_010281167.1	XRE family transcriptional regulator	Antitoxin
LJQ72_RS00115 (LJQ72_00115)	23760..24146	-	387	WP_039511562.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LJQ72_RS00120 (LJQ72_00120)	24468..25166	+	699	WP_005976717.1	D-galactonate utilization transcriptional regulator DgoR	-
LJQ72_RS00125 (LJQ72_00125)	25163..26059	+	897	WP_227527510.1	2-dehydro-3-deoxygalactonokinase	-
LJQ72_RS00130 (LJQ72_00130)	26043..26660	+	618	WP_010281152.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
LJQ72_RS00135 (LJQ72_00135)	26657..27805	+	1149	WP_010281150.1	galactonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-113)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14779.76 Da
• Isoelectric Point: 4.7491

>T220978 WP_039511562.1 NZ_CP085633:c24146-23760 [Pectobacterium brasiliense]
MEVYRLAWEIVTRALFDTWFDEQTETVQEEILAHLAILEEDGPQLGRPQVDQIKGSKYKNMKELRVQVGGHPFRTFFAFD
RTRKAIVLCAGDKKGEDEKLFYERMIKIADAEFASHLTSPEVKEDGDA

Nucleotide

• Length: 387 bp

>T220978 NZ_CP117322:3621922-3622197 [Salmonella enterica subsp. enterica serovar Derby]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGCGACTGGCTGGCAGAAGCGGGATTTGATACCGGGGCGAGCGTGACCGTGAAGAGCTCCGAGGGGTGTT
TGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATGATGTGGTGAACGGGAATTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11048.08 Da
• Isoelectric Point: 10.6442

>AT220978 WP_010281167.1 NZ_CP085633:c23773-23474 [Pectobacterium brasiliense]
MATLKEMMAKRSPESRARIEARTAEIRQEITLAKLREELNMSQTQLALALGVKQPSIVKMEKVENDPKLSTLKRYVTALG
GKLTLDVTLPDGKRIALTL

Nucleotide

• Length: 300 bp

>AT220978 NZ_CP117322:c3622187-3621775 [Salmonella enterica subsp. enterica serovar Derby]
TTCACCACATCATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGCTCTTCACGGTCACGCTCGCCCCGGTATCAAATCCCGCTT
CTGCCAGCCAGTCGCCCTTAAGGTGCTGGCTGGGGATTTGTGAGTAACAGGCGGTCATGCAGGTTTCGCTGTTGATGTGG
CGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTATTTATCCGCCGGATGCTGGCTGATTGTGGA
GCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACCGCGCCCCCCTCTCCCCGGCCAGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT

Structures

Antitoxin

• AlphaFold DB: A0A433N287