Detailed information of TA system TA220973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1832185..1832924
• Replicon: chromosome
• Accession: NZ_CP085627
• Organism: Erwinia rhapontici strain BY21311

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LJN55_RS08765
• Protein ID: WP_159337049.1
• Coordinates: 1832185..1832496 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJN55_RS08770
• Protein ID: WP_159337048.1
• Coordinates: 1832493..1832924 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJN55_RS08745 (LJN55_08745)	1827823..1828746	+	924	WP_133842968.1	phosphonate ABC transporter substrate-binding protein	-
LJN55_RS08750 (LJN55_08750)	1828810..1829679	+	870	WP_227536033.1	phosphonate ABC transporter, permease protein PhnE	-
LJN55_RS08755 (LJN55_08755)	1829676..1830560	+	885	WP_167864409.1	phosphonate ABC transporter, permease protein PhnE	-
LJN55_RS08760 (LJN55_08760)	1831348..1831986	-	639	WP_159337050.1	TetR family transcriptional regulator	-
LJN55_RS08765 (LJN55_08765)	1832185..1832496	+	312	WP_159337049.1	type II toxin-antitoxin system HigB family toxin	Toxin
LJN55_RS08770 (LJN55_08770)	1832493..1832924	+	432	WP_159337048.1	helix-turn-helix domain-containing protein	Antitoxin
LJN55_RS08775 (LJN55_08775)	1832936..1833700	-	765	WP_133842980.1	hypothetical protein	-
LJN55_RS08780 (LJN55_08780)	1833777..1834604	-	828	WP_227536034.1	AraC family transcriptional regulator	-
LJN55_RS08785 (LJN55_08785)	1834851..1836155	+	1305	WP_227536035.1	alpha-glucosidase/alpha-galactosidase	-
LJN55_RS08790 (LJN55_08790)	1836238..1837692	+	1455	WP_133842986.1	melibiose:sodium transporter MelB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-141)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12602.44 Da
• Isoelectric Point: 10.0379

>T220973 WP_159337049.1 NZ_CP085627:1832185-1832496 [Erwinia rhapontici]
MRVISRRPFIEAARCYPNAALSLDDIWRVLNSRDYITPEQLKAQFASLDRFKYRRKWWVINAAGNHLRIITYIKFETQNV
FIKHIVTHDEYDRLTDYYRRHPE

Nucleotide

• Length: 312 bp

>T220973 NZ_CP117322:817262-817675 [Salmonella enterica subsp. enterica serovar Derby]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGCCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAAAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15984.39 Da
• Isoelectric Point: 6.2193

>AT220973 WP_159337048.1 NZ_CP085627:1832493-1832924 [Erwinia rhapontici]
MRVAEKKFHTALSASAALIHAIPFLGRQATTHDYEDALVLVEYLIDHDDENPLIDILAKKISAWENVAPAFADFNARIDA
TPRELAVLRVLMDQHGLNQSSFQQEIGSRSLVSMIMSGQRALTLEHMRALAKRFSIPLATFIE

Nucleotide

• Length: 432 bp

>AT220973 NZ_CP117322:817015-817281 [Salmonella enterica subsp. enterica serovar Derby]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA