Detailed information of TA system TA220961

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 42680..42949
• Replicon: plasmid unnamed1
• Accession: NZ_CP085624
• Organism: Escherichia coli strain EC71

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LJN53_RS24755
• Protein ID: WP_001372321.1
• Coordinates: 42824..42949 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	42680..42745 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJN53_RS24710 (37702)	37702..37974	+	273	WP_072118979.1	hypothetical protein	-
LJN53_RS24715 (38215)	38215..38421	+	207	WP_000275858.1	hypothetical protein	-
LJN53_RS24720 (38447)	38447..38986	+	540	WP_001825195.1	single-stranded DNA-binding protein	-
LJN53_RS24725 (39049)	39049..39282	+	234	WP_000005990.1	DUF905 family protein	-
LJN53_RS24730 (39348)	39348..41306	+	1959	WP_065800285.1	ParB/RepB/Spo0J family partition protein	-
LJN53_RS24735 (41361)	41361..41795	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LJN53_RS24740 (41792)	41792..42554	+	763	Protein_49	plasmid SOS inhibition protein A	-
LJN53_RS24745 (42523)	42523..42711	-	189	WP_001299721.1	hypothetical protein	-
- (42680)	42680..42745	+	66	NuclAT_1	-	-
- (42680)	42680..42745	-	66	NuclAT_0	-	Antitoxin
- (42680)	42680..42745	-	66	NuclAT_0	-	Antitoxin
- (42523)	42523..42747	+	225	NuclAT_0	-	-
- (42523)	42523..42747	+	225	NuclAT_0	-	-
- (42523)	42523..42747	+	225	NuclAT_0	-	-
- (42523)	42523..42747	+	225	NuclAT_0	-	-
- (42523)	42523..42747	-	225	NuclAT_0	-	-
LJN53_RS24750 (42733)	42733..42882	+	150	Protein_51	plasmid maintenance protein Mok	-
LJN53_RS24755 (42824)	42824..42949	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LJN53_RS24760 (43169)	43169..43399	+	231	WP_001426396.1	hypothetical protein	-
LJN53_RS24765 (43397)	43397..43570	-	174	Protein_54	hypothetical protein	-
LJN53_RS24770 (43640)	43640..43846	+	207	WP_000547968.1	hypothetical protein	-
LJN53_RS24775 (43871)	43871..44158	+	288	WP_000107544.1	hypothetical protein	-
LJN53_RS24780 (44276)	44276..45097	+	822	WP_001234469.1	DUF932 domain-containing protein	-
LJN53_RS24785 (45394)	45394..46041	-	648	WP_031943493.1	transglycosylase SLT domain-containing protein	-
LJN53_RS24790 (46318)	46318..46701	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LJN53_RS24795 (46892)	46892..47578	+	687	WP_000332484.1	PAS domain-containing protein	-
LJN53_RS24800 (47672)	47672..47899	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T220961 WP_001372321.1 NZ_CP085624:42824-42949 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T220961 NZ_CP117317:c2306444-2306160 [Salmonella enterica subsp. enterica serovar Derby]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT220961 NZ_CP085624:c42745-42680 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin