Detailed information of TA system TA220959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4576207..4576809
• Replicon: chromosome
• Accession: NZ_CP085622
• Organism: Escherichia coli strain EC71

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LJN53_RS21965
• Protein ID: WP_000897305.1
• Coordinates: 4576498..4576809 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJN53_RS21960
• Protein ID: WP_000356397.1
• Coordinates: 4576207..4576497 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJN53_RS21935 (4572133)	4572133..4573035	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LJN53_RS21940 (4573032)	4573032..4573667	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LJN53_RS21945 (4573664)	4573664..4574593	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
LJN53_RS21950 (4574923)	4574923..4575165	-	243	WP_001087409.1	protein YiiF	-
LJN53_RS21955 (4575385)	4575385..4575603	-	219	WP_001315930.1	CopG family transcriptional regulator	-
LJN53_RS21960 (4576207)	4576207..4576497	-	291	WP_000356397.1	NadS family protein	Antitoxin
LJN53_RS21965 (4576498)	4576498..4576809	-	312	WP_000897305.1	hypothetical protein	Toxin
LJN53_RS21970 (4577038)	4577038..4577946	+	909	WP_001162704.1	alpha/beta hydrolase	-
LJN53_RS21975 (4578010)	4578010..4578951	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LJN53_RS21980 (4578996)	4578996..4579433	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
LJN53_RS21985 (4579430)	4579430..4580302	-	873	WP_000920762.1	virulence factor BrkB family protein	-
LJN53_RS21990 (4580296)	4580296..4580895	-	600	WP_001295269.1	glucose-1-phosphatase	-
LJN53_RS21995 (4580994)	4580994..4581779	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	aac(3)-IId / floR	-	4478835..4621663	142828

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T220959 WP_000897305.1 NZ_CP085622:c4576809-4576498 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T220959 NZ_CP117317:816356-816769 [Salmonella enterica subsp. enterica serovar Derby]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGCCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAAAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT220959 WP_000356397.1 NZ_CP085622:c4576497-4576207 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT220959 NZ_CP117317:816109-816375 [Salmonella enterica subsp. enterica serovar Derby]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7