Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3714847..3715388 Replicon chromosome
Accession NZ_CP085622
Organism Escherichia coli strain EC71

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag LJN53_RS17995 Protein ID WP_000615976.1
Coordinates 3715110..3715388 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag LJN53_RS17990 Protein ID WP_000729704.1
Coordinates 3714847..3715107 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJN53_RS17965 (3710630) 3710630..3711415 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
LJN53_RS17970 (3711387) 3711387..3713099 + 1713 Protein_3512 flagellar biosynthesis protein FlhA -
LJN53_RS17975 (3713204) 3713204..3713482 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
LJN53_RS17980 (3713475) 3713475..3713831 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
LJN53_RS17985 (3713888) 3713888..3714661 - 774 WP_000543895.1 C40 family peptidase -
LJN53_RS17990 (3714847) 3714847..3715107 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LJN53_RS17995 (3715110) 3715110..3715388 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LJN53_RS18000 (3715544) 3715544..3716284 + 741 WP_063100007.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LJN53_RS18005 (3716255) 3716255..3717022 - 768 WP_000333380.1 class II glutamine amidotransferase -
LJN53_RS18010 (3717228) 3717228..3717806 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3713475..3726174 12699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T220956 WP_000615976.1 NZ_CP085622:3715110-3715388 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T220956 NZ_CP117312:c4634181-4633870 [Salmonella enterica subsp. enterica serovar Derby]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGTTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGTGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT220956 WP_000729704.1 NZ_CP085622:3714847-3715107 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT220956 NZ_CP117312:c4633869-4633579 [Salmonella enterica subsp. enterica serovar Derby]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References