Detailed information of TA system TA220937

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 597287..598086
• Replicon: chromosome
• Accession: NZ_CP085622
• Organism: Escherichia coli strain EC71

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F4VJD3
• Locus tag: LJN53_RS02915
• Protein ID: WP_000347266.1
• Coordinates: 597287..597751 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: LJN53_RS02920
• Protein ID: WP_001307405.1
• Coordinates: 597751..598086 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJN53_RS02885 (592288)	592288..592722	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
LJN53_RS02890 (592740)	592740..593618	-	879	WP_001315856.1	PTS N-acetylgalactosamine transporter subunit IID	-
LJN53_RS02895 (593608)	593608..594387	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
LJN53_RS02900 (594398)	594398..594871	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
LJN53_RS02905 (594894)	594894..596174	-	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
LJN53_RS02910 (596423)	596423..597232	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
LJN53_RS02915 (597287)	597287..597751	-	465	WP_000347266.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
LJN53_RS02920 (597751)	597751..598086	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
LJN53_RS02925 (598235)	598235..599806	-	1572	WP_001273741.1	galactarate dehydratase	-
LJN53_RS02930 (600181)	600181..601515	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
LJN53_RS02935 (601531)	601531..602301	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	597287..609074	11787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17841.18 Da
• Isoelectric Point: 9.4947

>T220937 WP_000347266.1 NZ_CP085622:c597751-597287 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T220937 NZ_CP117307:c2312703-2312419 [Salmonella enterica subsp. enterica serovar Derby]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT220937 WP_001307405.1 NZ_CP085622:c598086-597751 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT220937 NZ_CP117307:c2312941-2312693 [Salmonella enterica subsp. enterica serovar Derby]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: A0A836NGD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7