Detailed information of TA system TA220931

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 42692..42956
• Replicon: plasmid pEC32-IncI1
• Accession: NZ_CP085621
• Organism: Escherichia coli strain EC32

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: LJN50_RS24905
• Protein ID: WP_001387489.1
• Coordinates: 42804..42956 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	42692..42752 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJN50_RS24890 (38794)	38794..39864	-	1071	WP_012783932.1	IncI1-type conjugal transfer protein TrbB	-
LJN50_RS24895 (39883)	39883..41091	-	1209	WP_001703845.1	IncI1-type conjugal transfer protein TrbA	-
- (41271)	41271..41328	-	58	NuclAT_1	-	-
- (41271)	41271..41328	-	58	NuclAT_1	-	-
- (41271)	41271..41328	-	58	NuclAT_1	-	-
- (41271)	41271..41328	-	58	NuclAT_1	-	-
LJN50_RS24900 (41398)	41398..42483	-	1086	WP_000080543.1	protein finQ	-
- (42692)	42692..42752	-	61	NuclAT_0	-	Antitoxin
- (42692)	42692..42752	-	61	NuclAT_0	-	Antitoxin
- (42692)	42692..42752	-	61	NuclAT_0	-	Antitoxin
- (42692)	42692..42752	-	61	NuclAT_0	-	Antitoxin
LJN50_RS24905 (42804)	42804..42956	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
LJN50_RS24910 (43028)	43028..43279	-	252	WP_001291964.1	hypothetical protein	-
LJN50_RS24915 (43579)	43579..43875	+	297	WP_011264046.1	DinQ-like type I toxin DqlB	-
LJN50_RS24920 (43940)	43940..44116	-	177	WP_001054900.1	hypothetical protein	-
LJN50_RS24925 (44508)	44508..44717	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
LJN50_RS24930 (44789)	44789..45451	-	663	WP_012783935.1	plasmid IncI1-type surface exclusion protein ExcA	-
LJN50_RS24935 (45522)	45522..47690	-	2169	WP_015508354.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55	-	1..88542	88542

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T220931 WP_001387489.1 NZ_CP085621:42804-42956 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T220931 NZ_CP117305:c4276896-4276405 [Salmonella enterica subsp. enterica serovar Derby]
ATGATCTCCCCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGCCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCGCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATCCCGATTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTACCAGCGGGTGGGGTTTGAACCATCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT220931 NZ_CP085621:c42752-42692 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin