Detailed information of TA system TA022092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1431721..1432318
• Replicon: chromosome
• Accession: NC_009483
• Organism: Geobacter uraniireducens Rf4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A5GAH0
• Locus tag: GURA_RS06205
• Protein ID: WP_011938141.1
• Coordinates: 1431721..1432002 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GURA_RS06210
• Protein ID: WP_011938142.1
• Coordinates: 1432013..1432318 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GURA_RS06190	1427264..1428589	+	1326	WP_011938138.1	electron transfer flavoprotein subunit alpha	-
GURA_RS06195	1428818..1430803	+	1986	WP_011938139.1	4Fe-4S dicluster domain-containing protein	-
GURA_RS06200	1430894..1431583	+	690	WP_011938140.1	type 1 glutamine amidotransferase	-
GURA_RS06205	1431721..1432002	+	282	WP_011938141.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GURA_RS06210	1432013..1432318	+	306	WP_011938142.1	HigA family addiction module antidote protein	Antitoxin
GURA_RS24140	1433131..1433817	+	687	WP_157046138.1	hypothetical protein	-
GURA_RS06215	1434056..1434916	+	861	WP_041245790.1	AraC family transcriptional regulator	-
GURA_RS06220	1435232..1435633	-	402	WP_011938144.1	type II toxin-antitoxin system HicB family antitoxin	-
GURA_RS23285	1435635..1435823	-	189	WP_083764790.1	type II toxin-antitoxin system HicA family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-98)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10854.22 Da
• Isoelectric Point: 7.1342

>T22092 WP_011938141.1 NC_009483:1431721-1432002 [Geobacter uraniireducens Rf4]
MINSFRCKETEKLFNGKFSSKLPQAIQRLAARKLDYLDGAGDLDDLRSPPGNRLETLKDDRAGQHSIRINDQWRICFVWR
DNNAFDVEIVDYH

Nucleotide

• Length: 282 bp

>T22092 NC_009483:1431721-1432002 [Geobacter uraniireducens Rf4]
ATGATCAATTCCTTTAGGTGCAAAGAAACTGAAAAGTTATTCAACGGCAAATTTTCCTCCAAGTTGCCACAGGCAATACA
GCGCCTTGCCGCTCGAAAACTTGACTATCTGGATGGTGCCGGGGATCTTGATGACTTGCGTTCCCCTCCCGGAAACCGTC
TGGAAACTTTAAAGGATGATCGCGCCGGACAACACAGCATTCGCATTAATGACCAGTGGCGCATCTGTTTTGTATGGCGT
GACAATAACGCCTTCGATGTCGAAATCGTTGACTACCATTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11621.51 Da
• Isoelectric Point: 9.5598

>AT22092 WP_011938142.1 NC_009483:1432013-1432318 [Geobacter uraniireducens Rf4]
MSKRDFPPIHPGEILREEFLIPLGISQYRLAKEIHVPARRINEIVLEKRGISADTALRLGRYFGTTAQLWINLQARYDLE
VARDAIEDKLEAEIKPLKRAA

Nucleotide

• Length: 306 bp

>AT22092 NC_009483:1432013-1432318 [Geobacter uraniireducens Rf4]
ATGAGCAAAAGAGATTTTCCTCCAATTCATCCTGGTGAAATCTTGCGCGAAGAATTTCTAATTCCGCTTGGTATCAGTCA
GTACCGACTCGCAAAGGAAATACACGTTCCGGCTCGTCGCATAAACGAGATCGTACTTGAAAAGCGTGGGATTTCCGCGG
ATACCGCGCTTCGCCTGGGACGCTATTTCGGCACGACGGCGCAGCTTTGGATTAACCTGCAAGCACGGTATGATCTTGAG
GTAGCGCGTGACGCGATTGAAGATAAGCTGGAAGCTGAAATTAAGCCATTGAAAAGGGCGGCATGA

Structures

Toxin

• AlphaFold DB: A5GAH0