Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) HipBST/HipA(toxin)
Location 186075..187393 Replicon chromosome
Accession NZ_CP085619
Organism Escherichia coli strain EC32

Toxin (Protein)


Gene name HipT Uniprot ID F4T5A3
Locus tag LJN50_RS00880 Protein ID WP_001262467.1
Coordinates 186386..187393 (+) Length 336 a.a.

Antitoxin (Protein)


Gene name HipS Uniprot ID F4T5A4
Locus tag LJN50_RS00875 Protein ID WP_001312177.1
Coordinates 186075..186386 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJN50_RS00850 (181607) 181607..182626 + 1020 WP_217823678.1 dipeptide ABC transporter permease DppB -
LJN50_RS00855 (182636) 182636..183538 + 903 WP_000084677.1 dipeptide ABC transporter permease DppC -
LJN50_RS00860 (183549) 183549..184532 + 984 WP_001196477.1 dipeptide ABC transporter ATP-binding protein -
LJN50_RS00865 (184529) 184529..185542 + 1014 WP_000103572.1 dipeptide ABC transporter ATP-binding subunit DppF -
LJN50_RS00870 (185767) 185767..186090 + 324 WP_000563102.1 helix-turn-helix transcriptional regulator -
LJN50_RS00875 (186075) 186075..186386 + 312 WP_001312177.1 HipA N-terminal domain-containing protein Antitoxin
LJN50_RS00880 (186386) 186386..187393 + 1008 WP_001262467.1 HipA domain-containing protein Toxin
LJN50_RS00885 (187410) 187410..188681 - 1272 WP_001545666.1 amino acid permease -
- (189043) 189043..189108 - 66 NuclAT_27 - -
- (189043) 189043..189108 - 66 NuclAT_27 - -
- (189043) 189043..189108 - 66 NuclAT_27 - -
- (189043) 189043..189108 - 66 NuclAT_27 - -
LJN50_RS00890 (189157) 189157..189264 + 108 WP_001295224.1 type I toxin-antitoxin system toxin Ldr family protein -
- (189526) 189526..189583 - 58 NuclAT_26 - -
- (189526) 189526..189583 - 58 NuclAT_26 - -
- (189526) 189526..189583 - 58 NuclAT_26 - -
- (189526) 189526..189583 - 58 NuclAT_26 - -
LJN50_RS00895 (189640) 189640..189747 + 108 WP_000170748.1 type I toxin-antitoxin system toxin Ldr family protein -
LJN50_RS00900 (190123) 190123..190230 + 108 WP_001295224.1 type I toxin-antitoxin system toxin Ldr family protein -
LJN50_RS00905 (190316) 190316..191995 - 1680 Protein_178 cellulose biosynthesis protein BcsG -
LJN50_RS00910 (191992) 191992..192183 - 192 WP_000988308.1 cellulose biosynthesis protein BcsF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(56-302)

Antitoxin

HipA, N-terminal subdomain 1

(6-98)


Sequences

Toxin        


Download         Length: 336 a.a.        Molecular weight: 38320.60 Da        Isoelectric Point: 5.5965

>T220907 WP_001262467.1 NZ_CP085619:186386-187393 [Escherichia coli]
MANCRILLTPLNERDEQRGYSTQGLKRLSGTAKLTPRLGFTRTQFVQELPRQQKGMSISGYQPKLQLVLDEGEFRVVDHQ
GNFILKPSPADFPGLAENEHATMTLMSRLGFDVPVHGLLSFAPQSEEELEYAFVIRRYDRDNKGLPVHQEQLDGAMQIMD
KYGKTGNDNEQYVSYETLARFLVAHVNDNIAFKIDLFRRIVYAWLLGNNDMHLRNFGLVYSDGLTPALAPVYDFVSVAPY
PEYFHSNYLALPLLTREEGGRELAPGFHSDYGEYIGQDFLLLGESMGLAPRLLEKLFQDIRKENAIVMETYEQSFMTQDH
IQAVLQCYRHRLGLL

Download         Length: 1008 bp

>T220907 NZ_CP117300:c4831818-4831063 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGGCGCCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTTGGCCGTCGCCACGAGCCAAGCCTGTTCTACGCCGCGCTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGTGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11821.47 Da        Isoelectric Point: 6.0609

>AT220907 WP_001312177.1 NZ_CP085619:186075-186386 [Escherichia coli]
MHRRVKVLLYGQVVGELSQNDSGFLFQYAPDYHGPAISISLPVAQRQFPSEMLHPYFASLAPEGWLRQRYSQIQHRDEND
LLGMLIDNGKNLLGAIQILPWEE

Download         Length: 312 bp

>AT220907 NZ_CP117300:c4832321-4831953 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCCGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGAGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3K2ZTB5

Antitoxin

Source ID Structure
AlphaFold DB A0A3L3HKE2

References