Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2228882..2229801 Replicon chromosome
Accession NZ_CP085532
Organism Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag LJF87_RS10445 Protein ID WP_003900449.1
Coordinates 2229196..2229801 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag LJF87_RS10440 Protein ID WP_003410124.1
Coordinates 2228882..2229187 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF87_RS10410 (LJF87_10405) 2223893..2225149 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -
LJF87_RS10415 (LJF87_10410) 2225503..2226078 + 576 WP_003410100.1 hypothetical protein -
LJF87_RS10420 (LJF87_10415) 2226075..2227115 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
LJF87_RS10425 (LJF87_10420) 2227357..2228076 + 720 WP_010950643.1 DUF433 domain-containing protein -
LJF87_RS10430 (LJF87_10425) 2228066..2228482 + 417 WP_003410114.1 hypothetical protein -
LJF87_RS10435 (LJF87_10430) 2228498..2228797 - 300 WP_003410120.1 hypothetical protein -
LJF87_RS10440 (LJF87_10435) 2228882..2229187 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
LJF87_RS10445 (LJF87_10440) 2229196..2229801 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LJF87_RS10450 (LJF87_10445) 2229826..2230185 - 360 WP_003410131.1 hypothetical protein -
LJF87_RS10455 (LJF87_10450) 2230345..2230977 - 633 WP_003913310.1 hypothetical protein -
LJF87_RS10460 (LJF87_10455) 2230974..2231210 - 237 WP_003410133.1 hypothetical protein -
LJF87_RS10465 (LJF87_10460) 2231417..2232313 - 897 WP_011799238.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T220882 WP_003900449.1 NZ_CP085532:c2229801-2229196 [Mycobacterium tuberculosis variant bovis BCG]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T220882 NZ_CP117298:3718339-3718596 [Mycobacterium tuberculosis variant bovis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTAGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT220882 WP_003410124.1 NZ_CP085532:c2229187-2228882 [Mycobacterium tuberculosis variant bovis BCG]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT220882 NZ_CP117298:3718067-3718342 [Mycobacterium tuberculosis variant bovis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References