Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2186375..2187243 Replicon chromosome
Accession NZ_CP085532
Organism Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag LJF87_RS10170 Protein ID WP_010886136.1
Coordinates 2186375..2186752 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag LJF87_RS10175 Protein ID WP_003409886.1
Coordinates 2186794..2187243 (+) Length 150 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF87_RS10120 (LJF87_10115) 2182164..2182616 - 453 WP_010950636.1 lipoprotein -
LJF87_RS10125 (LJF87_10120) 2182680..2183081 + 402 WP_003409869.1 hypothetical protein -
LJF87_RS10130 (LJF87_10125) 2183074..2183256 - 183 WP_003409870.1 hypothetical protein -
LJF87_RS10135 (LJF87_10130) 2183370..2183720 - 351 WP_003409871.1 hypothetical protein -
LJF87_RS10140 (LJF87_10135) 2183731..2184633 - 903 WP_003409874.1 hypothetical protein -
LJF87_RS10145 (LJF87_10140) 2184654..2184845 - 192 WP_003409876.1 hypothetical protein -
LJF87_RS10150 (LJF87_10145) 2184846..2185142 - 297 WP_003409877.1 hypothetical protein -
LJF87_RS10155 (LJF87_10150) 2185382..2185597 + 216 WP_003409878.1 antitoxin -
LJF87_RS10160 (LJF87_10155) 2185594..2185905 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
LJF87_RS10165 (LJF87_10160) 2185879..2186400 - 522 WP_010950637.1 hypothetical protein -
LJF87_RS10170 (LJF87_10165) 2186375..2186752 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
LJF87_RS10175 (LJF87_10170) 2186794..2187243 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
LJF87_RS10180 (LJF87_10175) 2187240..2187785 + 546 WP_003409891.1 SecB-like chaperone -
LJF87_RS10185 (LJF87_10180) 2187674..2188288 - 615 WP_003901296.1 hypothetical protein -
LJF87_RS10190 (LJF87_10185) 2188337..2188633 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
LJF87_RS10195 (LJF87_10190) 2188630..2188881 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
LJF87_RS10200 (LJF87_10195) 2188868..2189362 + 495 WP_003899099.1 hypothetical protein -
LJF87_RS10205 (LJF87_10200) 2189522..2189929 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
LJF87_RS10210 (LJF87_10205) 2189933..2190205 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LJF87_RS10215 (LJF87_10210) 2190238..2191458 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-113)

Antitoxin

(44-91)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T220877 WP_010886136.1 NZ_CP085532:2186375-2186752 [Mycobacterium tuberculosis variant bovis BCG]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T220877 NZ_CP117298:c3087128-3086736 [Mycobacterium tuberculosis variant bovis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT220877 WP_003409886.1 NZ_CP085532:2186794-2187243 [Mycobacterium tuberculosis variant bovis BCG]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT220877 NZ_CP117298:c3087340-3087125 [Mycobacterium tuberculosis variant bovis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU


Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References