Detailed information of TA system TA022083

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 909950..910565
• Replicon: chromosome
• Accession: NC_009456
• Organism: Vibrio cholerae O395

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: VC0395_RS04410
• Protein ID: WP_001162670.1
• Coordinates: 910278..910565 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: VC0395_RS04405
• Protein ID: WP_001232701.1
• Coordinates: 909950..910267 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VC0395_RS04375	905042..905497	-	456	WP_001245327.1	GNAT family N-acetyltransferase	-
VC0395_RS04380	905627..906502	-	876	WP_000259044.1	hypothetical protein	-
VC0395_RS04390	906949..907431	-	483	WP_001895003.1	hypothetical protein	-
VC0395_RS04395	907488..908318	-	831	WP_000056616.1	abortive infection family protein	-
VC0395_RS04400	908523..909263	-	741	WP_000469836.1	PhzF family phenazine biosynthesis protein	-
VC0395_RS20805	909487..909525	-	39	WP_106019119.1	hypothetical protein	-
VC0395_RS20065	909541..909666	-	126	WP_001944767.1	DUF645 family protein	-
VC0395_RS20970	909843..909953	-	111	WP_134814058.1	DUF3265 domain-containing protein	-
VC0395_RS04405	909950..910267	-	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
VC0395_RS04410	910278..910565	-	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VC0395_RS04415	910832..911012	-	181	Protein_865	DUF645 family protein	-
VC0395_RS04420	911437..911670	-	234	WP_032482776.1	DUF3709 domain-containing protein	-
VC0395_RS04425	912073..912291	-	219	WP_001917086.1	DUF3709 domain-containing protein	-
VC0395_RS04430	912601..913254	-	654	WP_000226874.1	hypothetical protein	-
VC0395_RS04435	913311..913820	-	510	WP_000405986.1	GNAT family N-acetyltransferase	-
VC0395_RS04440	914062..914304	+	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
VC0395_RS04445	914323..914640	+	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VC0395_RS20975	914834..914929	-	96	WP_012706049.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	833458..916350	82892

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T22083 WP_001162670.1 NC_009456:c910565-910278 [Vibrio cholerae O395]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T22083 NC_009456:c910565-910278 [Vibrio cholerae O395]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT22083 WP_001232701.1 NC_009456:c910267-909950 [Vibrio cholerae O395]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT22083 NC_009456:c910267-909950 [Vibrio cholerae O395]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36