Detailed information of TA system TA220807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4470799..4471401
• Replicon: chromosome
• Accession: NZ_CP085488
• Organism: Escherichia coli strain VCSW8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LJY68_RS21325
• Protein ID: WP_000897305.1
• Coordinates: 4471090..4471401 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJY68_RS21320
• Protein ID: WP_000356397.1
• Coordinates: 4470799..4471089 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJY68_RS21295 (4466725)	4466725..4467627	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LJY68_RS21300 (4467624)	4467624..4468259	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LJY68_RS21305 (4468256)	4468256..4469185	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
LJY68_RS21310 (4469515)	4469515..4469757	-	243	WP_001087409.1	protein YiiF	-
LJY68_RS21315 (4469976)	4469976..4470194	-	219	WP_001295676.1	CopG family transcriptional regulator	-
LJY68_RS21320 (4470799)	4470799..4471089	-	291	WP_000356397.1	NadS family protein	Antitoxin
LJY68_RS21325 (4471090)	4471090..4471401	-	312	WP_000897305.1	hypothetical protein	Toxin
LJY68_RS21330 (4471630)	4471630..4472538	+	909	WP_001299484.1	alpha/beta hydrolase	-
LJY68_RS21335 (4472602)	4472602..4473543	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
LJY68_RS21340 (4473588)	4473588..4474025	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
LJY68_RS21345 (4474022)	4474022..4474894	-	873	WP_000920762.1	virulence factor BrkB family protein	-
LJY68_RS21350 (4474888)	4474888..4475487	-	600	WP_001295269.1	glucose-1-phosphatase	-
LJY68_RS21355 (4475586)	4475586..4476371	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T220807 WP_000897305.1 NZ_CP085488:c4471401-4471090 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T220807 NZ_CP117293:c1390774-1390556 [Salmonella enterica subsp. enterica serovar Typhi]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT220807 WP_000356397.1 NZ_CP085488:c4471089-4470799 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT220807 NZ_CP117293:c1391176-1390802 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7