Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3639525..3640066 Replicon chromosome
Accession NZ_CP085488
Organism Escherichia coli strain VCSW8

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag LJY68_RS17535 Protein ID WP_000615982.1
Coordinates 3639788..3640066 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag LJY68_RS17530 Protein ID WP_000729704.1
Coordinates 3639525..3639785 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJY68_RS17505 (3634561) 3634561..3635700 + 1140 WP_032216948.1 flagellar type III secretion system protein FlhB -
LJY68_RS17510 (3635684) 3635684..3637777 + 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
LJY68_RS17515 (3637882) 3637882..3638160 + 279 WP_042099148.1 type II toxin-antitoxin system HicA family toxin -
LJY68_RS17520 (3638153) 3638153..3638509 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
LJY68_RS17525 (3638566) 3638566..3639339 - 774 WP_000543899.1 C40 family peptidase -
LJY68_RS17530 (3639525) 3639525..3639785 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LJY68_RS17535 (3639788) 3639788..3640066 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LJY68_RS17540 (3640222) 3640222..3640962 + 741 WP_227534316.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LJY68_RS17545 (3640933) 3640933..3641700 - 768 WP_000333380.1 class II glutamine amidotransferase -
LJY68_RS17550 (3641906) 3641906..3642484 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3638153..3650852 12699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T220805 WP_000615982.1 NZ_CP085488:3639788-3640066 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T220805 NZ_CP117293:822597-822995 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCTGAAATTCATGCTTGATACCAATACTTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCACCGG
AGCGTAATTTTGCAGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTACCGAACTGGCCCGCAAGGGAACACCTGTCGGGTCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT220805 WP_000729704.1 NZ_CP085488:3639525-3639785 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT220805 NZ_CP117293:822370-822597 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGCACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References