Detailed information of TA system TA220715

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 971669..971901
• Replicon: chromosome
• Accession: NZ_CP085294
• Organism: Enterococcus faecalis strain EFS108

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: C7CXQ5
• Locus tag: LIT97_RS04595
• Protein ID: WP_002355568.1
• Coordinates: 971669..971785 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	971696..971901 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT97_RS04585	967107..969365	+	2259	WP_033657650.1	DNA helicase PcrA	-
LIT97_RS04590	969491..971521	+	2031	WP_002375948.1	NAD-dependent DNA ligase LigA	-
LIT97_RS04595	971669..971785	+	117	WP_002355568.1	putative holin-like toxin	Toxin
-	971696..971901	-	206	-	-	Antitoxin
LIT97_RS04600	972103..972408	+	306	WP_002355569.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC	-
LIT97_RS04605	972408..973877	+	1470	WP_002358701.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA	-
LIT97_RS04610	973877..975307	+	1431	WP_002358703.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
LIT97_RS04615	975326..976414	+	1089	WP_002355572.1	diacylglycerol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (3-35)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4065.93 Da
• Isoelectric Point: 5.9482

>T220715 WP_002355568.1 NZ_CP085294:971669-971785 [Enterococcus faecalis]
MFLSVEAALGLMIGFATLVVTIIFGILALVLDNKNNRS

Nucleotide

• Length: 117 bp

>T220715 NZ_CP117255:2562257-2562547 [Rhizobium tumorigenes]
ATGGTTGAGAAATTGACCCCTTACGACCCAGCCGAAGATCTCGTCTCGGATGAGGCAATGGCAGTCTTCATGGGCGAAGC
GTTCGAGACGGGCGATGCCGGCTATATCGCCCACGCCCTTGGCGTGGTCGCGCGTGCAAAAGGCATGACGCAGATTGCCA
CCGAAACCGGCCTGTCGCGGGAGCAACTCTACAGGTCTTTCAGTGAGGCAGGAAATCCAACCCTTAAGACAACGATGGCA
GTGATGAAGGCGCTCGGCATTACTCTCACCGCGAAGGTCGCAGCCGAATAA

Antitoxin

Nucleotide

• Length: 206 bp

>AT220715 NZ_CP085294:c971901-971696 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAGTAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Structures

Toxin

• AlphaFold DB: A0A1B4XLM6

Antitoxin