Detailed information of TA system TA220704

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 361411..361682
• Replicon: chromosome
• Accession: NZ_CP085294
• Organism: Enterococcus faecalis strain EFS108

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: A0A3N3Z2N6
• Locus tag: LIT97_RS01655
• Protein ID: WP_073340360.1
• Coordinates: 361411..361551 (+)
• Length: 47 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	361484..361682 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT97_RS01635	356720..357712	+	993	WP_002394775.1	biotin--[acetyl-CoA-carboxylase] ligase	-
LIT97_RS01640	357879..358517	-	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
LIT97_RS01645	359203..360819	+	1617	WP_002372620.1	phosphatase PAP2/LCP family protein	-
LIT97_RS01650	361149..361292	+	144	WP_002392818.1	type I toxin-antitoxin system toxin PepG1	-
LIT97_RS01655	361411..361551	+	141	WP_073340360.1	putative holin-like toxin	Toxin
-	361484..361682	-	199	-	-	Antitoxin
LIT97_RS01660	361782..361925	+	144	WP_002381035.1	putative holin-like toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (14-46)

Sequences

Toxin

Protein

• Length: 47 a.a.
• Molecular weight: 5218.33 Da
• Isoelectric Point: 10.3265

>T220704 WP_073340360.1 NZ_CP085294:361411-361551 [Enterococcus faecalis]
ISLKNTNKNIVYCTTYETIQTILGFGMFTIALIALIVKLLKNDKKK

Nucleotide

• Length: 141 bp

>T220704 NZ_CP117255:182819-183148 [Rhizobium tumorigenes]
ATGGAGCGAGGTGAGATCTGGTTTGTCGATCTGGAGCCGACACGCGGGCGCGAGCAAGCGAATGAGCGTTACGCTATGAT
CTTGTCCCCGAAGGTCGTCAACCAGCACGGAACCCAGATCGTCGCGCCTATCACCACCGGCGGTCAGTTTGCCAGGACGT
CCGGCTTTGCCGTCTCGCTGTCAGGCGCTGGAACGCGATCGACGGGAGTGGTGCTGTGCCACCAGATCCGCGCCGTCGAT
ATCAAAGCAAGACGGGGCCGTTTCGTGGAGAAGGCACCAGACTTCATCGTTGACGAGGTCTTGGCCAGGGTCGCGGCGCT
ATTCGACTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT220704 NZ_CP085294:c361682-361484 [Enterococcus faecalis]
TTTTTAGGTAATTGTGCTATAATGGTAATGAAAAGAGAGCTATGCTGTAACATACCTCTCTGATGTAGAGCCGTTTAAGA
CGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATT
TCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: A0A3N3Z2N6

Antitoxin