Detailed information of TA system TA220660

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 1721663..1721895
• Replicon: chromosome
• Accession: NZ_CP085289
• Organism: Enterococcus faecalis strain EFS17

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: C7CXQ5
• Locus tag: LIT95_RS08300
• Protein ID: WP_002355568.1
• Coordinates: 1721663..1721779 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	1721690..1721895 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT95_RS08290	1717101..1719359	+	2259	WP_002363179.1	DNA helicase PcrA	-
LIT95_RS08295	1719485..1721515	+	2031	WP_002358699.1	NAD-dependent DNA ligase LigA	-
LIT95_RS08300	1721663..1721779	+	117	WP_002355568.1	putative holin-like toxin	Toxin
-	1721690..1721895	-	206	-	-	Antitoxin
LIT95_RS08305	1722097..1722402	+	306	WP_002355569.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC	-
LIT95_RS08310	1722402..1723871	+	1470	WP_002358701.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA	-
LIT95_RS08315	1723871..1725301	+	1431	WP_002358703.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
LIT95_RS08320	1725320..1726408	+	1089	WP_002355572.1	diacylglycerol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (3-35)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4065.93 Da
• Isoelectric Point: 5.9482

>T220660 WP_002355568.1 NZ_CP085289:1721663-1721779 [Enterococcus faecalis]
MFLSVEAALGLMIGFATLVVTIIFGILALVLDNKNNRS

Nucleotide

• Length: 117 bp

>T220660 NZ_CP117235:c3093231-3092854 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Nucleotide

• Length: 206 bp

>AT220660 NZ_CP085289:c1721895-1721690 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAATAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Structures

Toxin

• AlphaFold DB: A0A1B4XLM6

Antitoxin