Detailed information of TA system TA022066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 293791..294402
• Replicon: chromosome
• Accession: NC_009446
• Organism: Dichelobacter nodosus VCS1703A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A5EWB1
• Locus tag: DNO_RS01325
• Protein ID: WP_012030619.1
• Coordinates: 294127..294402 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: DNO_RS01320
• Protein ID: WP_012030618.1
• Coordinates: 293791..294117 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DNO_RS01290 (DNO_0265)	289759..290040	-	282	WP_012030611.1	virulence protein	-
DNO_RS01295 (DNO_0266)	290906..292216	-	1311	WP_012030612.1	virulence-associated E family protein	-
DNO_RS01300 (DNO_0267)	292206..292553	-	348	WP_012030613.1	hypothetical protein	-
DNO_RS01305 (DNO_0269)	292557..292778	-	222	WP_012030615.1	hypothetical protein	-
DNO_RS01310 (DNO_0271)	293128..293475	+	348	WP_012030616.1	HigA family addiction module antidote protein	-
DNO_RS01315 (DNO_0270)	293468..293647	-	180	WP_041729402.1	hypothetical protein	-
DNO_RS01320 (DNO_0272)	293791..294117	-	327	WP_012030618.1	HigA family addiction module antidote protein	Antitoxin
DNO_RS01325 (DNO_0273)	294127..294402	-	276	WP_012030619.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DNO_RS01330 (DNO_0274)	295382..295660	+	279	WP_041729404.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DNO_RS01335 (DNO_0275)	295673..295981	+	309	WP_012030621.1	HigA family addiction module antidote protein	-
DNO_RS01340 (DNO_0276)	296317..296547	+	231	WP_012030622.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
DNO_RS01345 (DNO_0277)	296550..296951	+	402	WP_119185609.1	type II toxin-antitoxin system VapC family toxin	-
DNO_RS01350 (DNO_0278)	297073..297354	-	282	WP_012030611.1	virulence protein	-
DNO_RS01355 (DNO_0279)	297992..299305	-	1314	WP_012030624.1	virulence-associated E family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	282708..307332	24624

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-91)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-68)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10629.96 Da
• Isoelectric Point: 7.9047

>T22066 WP_012030619.1 NC_009446:c294402-294127 [Dichelobacter nodosus VCS1703A]
VIHSFADKDTYDFFHGTICKRFESFASVARRKLTMLDNAATLEFLRSPPANRLESLKGDRAGQYSIRINDRYRICFRWDS
GAHDVEIVDYH

Nucleotide

• Length: 276 bp

>T22066 NC_009446:c294402-294127 [Dichelobacter nodosus VCS1703A]
GTGATTCATAGTTTTGCCGATAAAGATACCTATGATTTCTTTCATGGAACAATTTGCAAACGCTTTGAGTCTTTTGCAAG
TGTCGCGCGGCGAAAGCTAACGATGCTTGATAATGCAGCAACTTTAGAGTTTTTACGTTCACCGCCAGCCAACCGTTTAG
AGTCACTAAAAGGAGATCGTGCAGGGCAATATAGTATTCGTATCAACGATCGTTACCGTATTTGCTTCCGTTGGGATAGT
GGCGCGCATGATGTGGAAATTGTTGATTATCATTAG

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12026.84 Da
• Isoelectric Point: 6.7258

>AT22066 WP_012030618.1 NC_009446:c294117-293791 [Dichelobacter nodosus VCS1703A]
MSIPAGKNEMPAIHPGEILREEYLKPMGLSAHALAKALHVSPSRINEIVREQRGITADTALRLVRYFGGDAQSWLNMQTA
YDLKMAEQDKQSINIIIPLSTDARNVEL

Nucleotide

• Length: 327 bp

>AT22066 NC_009446:c294117-293791 [Dichelobacter nodosus VCS1703A]
ATGAGTATTCCAGCTGGAAAAAATGAAATGCCAGCAATACACCCAGGCGAAATTTTGCGTGAAGAATATTTAAAACCGAT
GGGGCTTTCGGCGCATGCTTTAGCAAAGGCTTTGCATGTTTCTCCCTCACGTATTAACGAAATTGTACGTGAGCAACGAG
GCATTACGGCTGACACGGCATTACGTTTGGTGCGTTATTTTGGTGGTGATGCGCAAAGTTGGCTAAATATGCAAACAGCA
TACGATTTAAAGATGGCTGAACAAGACAAACAAAGCATTAATATCATCATTCCTTTAAGTACAGACGCAAGAAACGTTGA
ACTTTGA

Structures

Toxin

• AlphaFold DB: A5EWB1