Detailed information of TA system TA220653

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 1056271..1056576
• Replicon: chromosome
• Accession: NZ_CP085289
• Organism: Enterococcus faecalis strain EFS17

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: A0A6I4Y3R3
• Locus tag: LIT95_RS05070
• Protein ID: WP_033624079.1
• Coordinates: 1056271..1056414 (+)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	1056346..1056576 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT95_RS05050	1051484..1051699	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
LIT95_RS05055	1051838..1052830	+	993	WP_002358973.1	biotin--[acetyl-CoA-carboxylase] ligase	-
LIT95_RS05060	1052997..1053635	-	639	WP_126254829.1	lytic polysaccharide monooxygenase	-
LIT95_RS05065	1054322..1055938	+	1617	WP_126254830.1	phosphatase PAP2/LCP family protein	-
LIT95_RS05070	1056271..1056414	+	144	WP_033624079.1	putative holin-like toxin	Toxin
-	1056346..1056576	-	231	-	-	Antitoxin
LIT95_RS05075	1056608..1059667	-	3060	WP_161970875.1	WxL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (14-47)

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5186.29 Da
• Isoelectric Point: 10.5719

>T220653 WP_033624079.1 NZ_CP085289:1056271-1056414 [Enterococcus faecalis]
MNVSAKIYERRGLLSIAEALALMISFGSFIATLIFGILKAVKEDKKK

Nucleotide

• Length: 144 bp

>T220653 NZ_CP117235:1919636-1919890 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Nucleotide

• Length: 231 bp

>AT220653 NZ_CP085289:c1056576-1056346 [Enterococcus faecalis]
AGGCTCGGGCATTTTTATTGATGGTCTTTTGATGGTCGTTGTGCTATAATAGCATTGAAAAGAGAGATATGCTGTAACAT
ACCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATTTATAAAAAATAACCGTACTCGCTAAAGTAGAC
GGTTATTTTTTCTTGTCTTCTTTAACAGCTTTTAGGATTCCGAAGATTAACGTTGCGATAAAAGAGCCGAA

Structures

Toxin

• AlphaFold DB: A0A6I4Y3R3

Antitoxin