Detailed information of TA system TA220625

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2438515..2438740
• Replicon: chromosome
• Accession: NZ_CP085202
• Organism: Shigella flexneri strain P099

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: IUX27_RS12180
• Protein ID: WP_000813254.1
• Coordinates: 2438515..2438670 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2438682..2438740 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUX27_RS12145	2433641..2434201	-	561	WP_072075205.1	ORF6N domain-containing protein	-
IUX27_RS12155	2435412..2435585	-	174	WP_000504450.1	hypothetical protein	-
IUX27_RS12160	2435738..2436292	-	555	WP_000640143.1	DUF1133 family protein	-
IUX27_RS12165	2436289..2436579	-	291	WP_000228038.1	DUF1364 domain-containing protein	-
IUX27_RS12170	2436579..2437178	-	600	WP_000940329.1	DUF1367 family protein	-
IUX27_RS12175	2437312..2438009	+	698	WP_223368647.1	IS1 family transposase	-
IUX27_RS12180	2438515..2438670	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2438682..2438740	+	59	-	-	Antitoxin
IUX27_RS12185	2439125..2439490	-	366	WP_000610655.1	DUF551 domain-containing protein	-
IUX27_RS12190	2439490..2440155	-	666	WP_000208062.1	hypothetical protein	-
IUX27_RS12195	2440152..2440517	-	366	WP_001229297.1	HNH endonuclease signature motif containing protein	-
IUX27_RS12200	2440519..2440737	-	219	WP_000256998.1	DUF4014 family protein	-
IUX27_RS12205	2440830..2441186	-	357	WP_005048249.1	hypothetical protein	-
IUX27_RS12210	2441244..2441666	-	423	WP_001118167.1	DUF977 family protein	-
IUX27_RS12215	2441681..2442427	-	747	WP_000788996.1	ATP-binding protein	-
IUX27_RS12220	2442573..2442742	-	170	Protein_2364	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	2383664..2446657	62993

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T220625 WP_000813254.1 NZ_CP085202:c2438670-2438515 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T220625 NZ_CP117227:324617-324985 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT220625 NZ_CP085202:2438682-2438740 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin