Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 693111..693838 Replicon chromosome
Accession NZ_CP085202
Organism Shigella flexneri strain P099

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag IUX27_RS03310 Protein ID WP_000550189.1
Coordinates 693111..693425 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IUX27_RS03315 Protein ID WP_000560266.1
Coordinates 693422..693838 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUX27_RS03290 (689256) 689256..690254 - 999 WP_005050984.1 Gfo/Idh/MocA family oxidoreductase -
IUX27_RS03295 (690333) 690333..691025 - 693 WP_000942538.1 vancomycin high temperature exclusion protein -
IUX27_RS03300 (691102) 691102..691605 - 504 WP_005050988.1 M48 family metallopeptidase -
IUX27_RS03305 (691690) 691690..692826 + 1137 WP_000018656.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
IUX27_RS03310 (693111) 693111..693425 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
IUX27_RS03315 (693422) 693422..693838 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
IUX27_RS03320 (693883) 693883..695901 - 2019 WP_248501080.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
IUX27_RS03325 (696127) 696127..698478 - 2352 WP_000695506.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T220611 WP_000550189.1 NZ_CP085202:693111-693425 [Shigella flexneri]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T220611 NZ_CP117202:c3842-3576 [Pantoea sp. SS70]
TTGGTCTGGACGATTAATTACTCTGACCGTGCACTGAAAGCGTTACGTAAGATGGATAAGCAGAATGCGCGACGAATAGT
GGATTTTATGGACATGCGCATTGCTGTTTCGGACGATCCGCGTCAGTCAGGAAAACCGCTCAAGGGGGAGCTTGGTGAAT
TCTGGCGTTACCGTGTCGGTGATTATCGAATCCTATGTGAAATCAGAGATGACGAACTGGTGATTCTTGCCGCGACGATA
GGACATCGGCGTGATGTCTACGAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT220611 WP_000560266.1 NZ_CP085202:693422-693838 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT220611 NZ_CP117202:c4050-3826 [Pantoea sp. SS70]
ATGTTAGCTATCCGGCTGTCTGATGAAATCGAGTCAAGACTCGACTCGCTGGCAAAACAAACGGGCAGGACAAAGACGTT
CTACGCGCGGGAAGCGATCCTGACTCATCTGGAAGATTTAGAAGATTATTACCTAGCGGCGGAAACCGTTACACGTATAA
GGCGCGGAGAGGAATTCGTGCATTCGTCCGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References