Detailed information of TA system TA220589

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53309..53548
• Replicon: plasmid pEC195-93k
• Accession: NZ_CP085190
• Organism: Escherichia coli strain EC195

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: LIN52_RS25460
• Protein ID: WP_023144756.1
• Coordinates: 53309..53443 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	53488..53548 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN52_RS25430 (49010)	49010..49867	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
LIN52_RS25435 (49860)	49860..49934	-	75	WP_031943482.1	RepA leader peptide Tap	-
LIN52_RS25440 (49934)	49934..50065	-	132	Protein_64	protein CopA/IncA	-
LIN52_RS25445 (50296)	50296..51837	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
LIN52_RS25450 (51852)	51852..52598	+	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-
LIN52_RS25455 (52758)	52758..53012	-	255	WP_000083850.1	replication regulatory protein RepA	-
LIN52_RS25460 (53309)	53309..53443	-	135	WP_023144756.1	Hok/Gef family protein	Toxin
- (53488)	53488..53548	+	61	NuclAT_0	-	Antitoxin
- (53488)	53488..53548	+	61	NuclAT_0	-	Antitoxin
- (53488)	53488..53548	+	61	NuclAT_0	-	Antitoxin
- (53488)	53488..53548	+	61	NuclAT_0	-	Antitoxin
LIN52_RS25465 (53515)	53515..53801	-	287	Protein_69	DUF2726 domain-containing protein	-
LIN52_RS25470 (54314)	54314..54526	-	213	WP_013023861.1	hypothetical protein	-
LIN52_RS25475 (54657)	54657..55217	-	561	WP_000139341.1	fertility inhibition protein FinO	-
LIN52_RS25480 (55272)	55272..56018	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
LIN52_RS25485 (56038)	56038..58431	-	2394	Protein_73	conjugative transfer relaxase/helicase TraI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / sul1 / qacE / aadA2 / dfrA12 / blaNDM-5 / aadA5 / blaTEM-1B / aac(3)-IId / mph(A) / aph(6)-Id / aph(3'')-Ib / sul2	-	1..93120	93120

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T220589 WP_023144756.1 NZ_CP085190:c53443-53309 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T220589 NZ_CP117194:c41809-41405 [Acidovorax temperans]
ATGTTCATGCTGGACACGGATACGTGCATCTTCCTCATGCGAGGTGAGTCCCAGACGCTGGCGGTTAAGGTTCAGTCGGT
GCCTCTACAGCAACAGGTGATGTCAGCTGTGACGTTCGCCGAACTGACCTATGGCGTGCAGGCTTCCGCCGTCGCTAAGC
GCAAACAGAATCAAGCCGTTCTCGATAGTTTGGCTTTGCACTTGGCTGTTCTGGACTGGCCTCAAGAGGCGGCGAAACAC
TACGCCGAGATTCGTGCCGATCTAAAAAAACGAGGCGCGCAGCTCGGCGCTGCTGATCTCATGATTGCGGCACATGCGCG
CGCCATGGGAGCCGTCATCGTCACGAACAATGTGAAGGACTTCGGGCGCGTGAAGGGCCTGCAAGTCGAGAACTGGACTA
AATAG

Antitoxin

Nucleotide

• Length: 61 bp

>AT220589 NZ_CP085190:53488-53548 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin