Detailed information of TA system TA220570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1111669..1112396
• Replicon: chromosome
• Accession: NZ_CP085188
• Organism: Escherichia coli strain EC195

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: LIN52_RS05430
• Protein ID: WP_000547564.1
• Coordinates: 1111669..1111980 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LIN52_RS05435
• Protein ID: WP_000126294.1
• Coordinates: 1111977..1112396 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN52_RS05400 (1106811)	1106811..1108520	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
LIN52_RS05405 (1108530)	1108530..1109072	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
LIN52_RS05410 (1109072)	1109072..1109839	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
LIN52_RS05415 (1109836)	1109836..1110246	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
LIN52_RS05420 (1110239)	1110239..1110709	+	471	WP_060621146.1	hydrogenase maturation peptidase HycI	-
LIN52_RS05425 (1110734)	1110734..1111495	+	762	WP_001026446.1	hypothetical protein	-
LIN52_RS05430 (1111669)	1111669..1111980	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
LIN52_RS05435 (1111977)	1111977..1112396	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
LIN52_RS05440 (1112510)	1112510..1113934	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
LIN52_RS05445 (1113943)	1113943..1115400	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
LIN52_RS05450 (1115660)	1115660..1116670	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
LIN52_RS05455 (1116819)	1116819..1117346	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T220570 WP_000547564.1 NZ_CP085188:1111669-1111980 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T220570 NZ_CP117181:c3769505-3769194 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGCATGTCATATCAAAAGAGCCTTTTGATGAAGCGGCAAGACGATATCCCAATGACTCGTTAGCCATAAAAGCCCTGTA
TCGCTTAGTCCGCGAAAGAGATTTCTCTTCTCCGGCAGAGCTACGAAAAGTCATACCGAGTCTCGATAACTTTAAGTACC
GTAACAAGTGGTGGGTACTGGATGTCGGAGGCAACAACCTGAGGGTGATCGCTTACATCAACTTTATAAACAAACGCTTT
TATGTGAAACACATCGCCACTCACGCTGAATATGACAAGCTGACCCGCTACTACAGGGAGAATAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT220570 WP_000126294.1 NZ_CP085188:1111977-1112396 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT220570 NZ_CP117181:c3769197-3768778 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGATTGCCAACACCGCAAAAGCTATCGAAGCCACAAAGGCGCTGGTCGCCGCCGTTCCTTTCCTGGGTGGCAGCGCGTC
AGAGAAAGATTATCGTGATGCGCTAGCGCTGGTTGATTATCTTATCGAAAATGACGATGAAAACCCGCTGATTGACTTCC
TGGCAAGCAAAATTGCCGAGTACGAAGATAACAGCAAGCAGTTCGCCGAGTTCAACAAATCTGTTGCTGAAATGCCGGTT
GGTGTTGCCCTGCTGCGCACGCTAATTGACCAGTACAAACTCTCATACTCAGATCTTAAAGAAGAAATCGGTTCCAAATC
ACTGGTAAGTCAGATCCTGTCCGGACAGCGCTCGTTAACGATTACGCACATCAAAGCGCTTTCAGCACGATTTGGCGTGA
AACCTGAGTGGTTCTTATAA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5