Detailed information of TA system TA220567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 769710..770403
• Replicon: chromosome
• Accession: NZ_CP085188
• Organism: Escherichia coli strain EC195

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: U9ZN09
• Locus tag: LIN52_RS03765
• Protein ID: WP_000415585.1
• Coordinates: 769710..770006 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: LIN52_RS03770
• Protein ID: WP_000650107.1
• Coordinates: 770008..770403 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN52_RS03730 (764844)	764844..765323	+	480	WP_000065332.1	Hcp family type VI secretion system effector	-
LIN52_RS03735 (765326)	765326..766036	+	711	WP_000834030.1	hypothetical protein	-
LIN52_RS03740 (766043)	766043..766375	+	333	WP_000914690.1	DUF2645 family protein	-
LIN52_RS03745 (766421)	766421..767770	-	1350	WP_000673358.1	quorum sensing histidine kinase QseC	-
LIN52_RS03750 (767767)	767767..768426	-	660	WP_001221502.1	quorum sensing response regulator transcription factor QseB	-
LIN52_RS03755 (768578)	768578..768970	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
LIN52_RS03760 (769023)	769023..769505	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
LIN52_RS03765 (769710)	769710..770006	+	297	WP_000415585.1	type II toxin-antitoxin system toxin MqsR	Toxin
LIN52_RS03770 (770008)	770008..770403	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
LIN52_RS03775 (770536)	770536..772143	+	1608	WP_001375094.1	ABC transporter substrate-binding protein	-
LIN52_RS03780 (772281)	772281..774539	+	2259	WP_001281841.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (2-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11261.99 Da
• Isoelectric Point: 8.9070

>T220567 WP_000415585.1 NZ_CP085188:769710-770006 [Escherichia coli]
MEKRTPHTRLSQVKKLVNTGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T220567 NZ_CP117181:2592307-2592720 [Salmonella enterica subsp. enterica serovar Mbandaka]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCATCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT220567 WP_000650107.1 NZ_CP085188:770008-770403 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT220567 NZ_CP117181:2592060-2592326 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: U9ZN09

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65