Detailed information of TA system TA220561

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 48610..48863
• Replicon: plasmid pEC7518-49k
• Accession: NZ_CP085186
• Organism: Escherichia coli strain EC7518

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LIN51_RS25290
• Protein ID: WP_001312851.1
• Coordinates: 48714..48863 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	48610..48669 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN51_RS25265 (45337)	45337..46083	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
LIN51_RS25270 (46138)	46138..46698	+	561	WP_000139328.1	fertility inhibition protein FinO	-
LIN51_RS25275 (46830)	46830..47030	+	201	WP_015059022.1	hypothetical protein	-
LIN51_RS25280 (47416)	47416..48015	+	600	WP_032083981.1	PIN domain-containing protein	-
LIN51_RS25285 (48077)	48077..48409	+	333	WP_152916585.1	hypothetical protein	-
- (48610)	48610..48669	-	60	NuclAT_1	-	Antitoxin
- (48610)	48610..48669	-	60	NuclAT_1	-	Antitoxin
- (48610)	48610..48669	-	60	NuclAT_1	-	Antitoxin
- (48610)	48610..48669	-	60	NuclAT_1	-	Antitoxin
LIN51_RS25290 (48714)	48714..48863	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
LIN51_RS25295 (49147)	49147..49395	+	249	WP_000083837.1	replication regulatory protein RepA	-
LIN51_RS25300 (49510)	49510..49694	+	185	Protein_64	protein CopA/IncA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / blaTEM-1B / rmtB	-	1..49706	49706

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T220561 WP_001312851.1 NZ_CP085186:48714-48863 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T220561 NZ_CP117181:c1241853-1241362 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGATCTCCTCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCGCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGATTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTACCAGCGGGTGGGGTTTGAACCATCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT220561 NZ_CP085186:c48669-48610 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin