Detailed information of TA system TA220554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4743189..4743791
• Replicon: chromosome
• Accession: NZ_CP085183
• Organism: Escherichia coli strain EC7518

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LIN51_RS22955
• Protein ID: WP_000897305.1
• Coordinates: 4743480..4743791 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LIN51_RS22950
• Protein ID: WP_000356397.1
• Coordinates: 4743189..4743479 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN51_RS22925 (4739134)	4739134..4740036	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LIN51_RS22930 (4740033)	4740033..4740668	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LIN51_RS22935 (4740665)	4740665..4741594	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
LIN51_RS22940 (4741924)	4741924..4742166	-	243	WP_001087409.1	protein YiiF	-
LIN51_RS22945 (4742385)	4742385..4742603	-	219	WP_001295676.1	CopG family transcriptional regulator	-
LIN51_RS22950 (4743189)	4743189..4743479	-	291	WP_000356397.1	NadS family protein	Antitoxin
LIN51_RS22955 (4743480)	4743480..4743791	-	312	WP_000897305.1	hypothetical protein	Toxin
LIN51_RS22960 (4744020)	4744020..4744928	+	909	WP_001162704.1	alpha/beta hydrolase	-
LIN51_RS22965 (4744992)	4744992..4745933	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LIN51_RS22970 (4745978)	4745978..4746415	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
LIN51_RS22975 (4746412)	4746412..4747284	-	873	WP_000920762.1	virulence factor BrkB family protein	-
LIN51_RS22980 (4747278)	4747278..4747877	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T220554 WP_000897305.1 NZ_CP085183:c4743791-4743480 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T220554 NZ_CP117181:310978-311196 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT220554 WP_000356397.1 NZ_CP085183:c4743479-4743189 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT220554 NZ_CP117181:310576-310950 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7