Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3899719..3900260 Replicon chromosome
Accession NZ_CP085183
Organism Escherichia coli strain EC7518

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag LIN51_RS18990 Protein ID WP_000615982.1
Coordinates 3899982..3900260 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag LIN51_RS18985 Protein ID WP_000729704.1
Coordinates 3899719..3899979 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIN51_RS18960 (3894755) 3894755..3895894 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
LIN51_RS18965 (3895878) 3895878..3897971 + 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
LIN51_RS18970 (3898076) 3898076..3898354 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
LIN51_RS18975 (3898347) 3898347..3898703 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
LIN51_RS18980 (3898760) 3898760..3899533 - 774 WP_000543899.1 C40 family peptidase -
LIN51_RS18985 (3899719) 3899719..3899979 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LIN51_RS18990 (3899982) 3899982..3900260 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LIN51_RS18995 (3900416) 3900416..3901156 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LIN51_RS19000 (3901127) 3901127..3901894 - 768 WP_000333380.1 class II glutamine amidotransferase -
LIN51_RS19005 (3902100) 3902100..3902678 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T220551 WP_000615982.1 NZ_CP085183:3899982-3900260 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T220551 NZ_CP117168:5247985-5248263 [Serratia proteamaculans]
GTGAAGCTCAAGTGGACGGAACCCGCGATTGCCGACCGTATGGCAATTTATGACTATCTGGCATCAAAGAATCCAGTCGC
TGCCGCAGAAATTGACGCCCTGATTTCCGCGGCTACGGCCCGCCTCTCCCAAAACCCATTGAGCGGCAAACCAGGCCGGG
CTCCAGGTACCCGTGAGCTAGTCATTCATCATTCTTACTTGCTGATTTATGAATTAACCAACGGCCCGCTTATCATATTG
GCGGTAGTCCACGCCCGCCGCCAATGGCCAAAAGATTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT220551 WP_000729704.1 NZ_CP085183:3899719-3899979 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT220551 NZ_CP117168:5247698-5247967 [Serratia proteamaculans]
ATGTCTGAATCTACCGTGGTGATCCGAGTGGATGATGAATTAAAAATGGCTTTCGCCAGCGCGGCAAAAGCAGCAGATCG
TACTGCATCTCAACTGTTACGTGATTTCATGCGGGATTTTGTTTGTCGCCAATCTCAGCAAAAAGAATACGAGCAATGGC
TACAGGAAAAAGTGGAAATAAGCCGGAAAGCACTTCATGAAGGGAAAATCGTTGATGATGAAGATGTTGAGGCTTACTTC
GCCGAACGCAGGGAAAAATCAATGCGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References