Detailed information of TA system TA220529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 202772..203286
• Replicon: plasmid pDSM110093_b
• Accession: NZ_CP085169
• Organism: Sulfitobacter sp. DSM 110093

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: DSM110093_RS19105
• Protein ID: WP_243267797.1
• Coordinates: 202772..203038 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: DSM110093_RS19110
• Protein ID: WP_243267798.1
• Coordinates: 203035..203286 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSM110093_RS19090 (DSM110093_03888)	199008..200153	-	1146	WP_243267794.1	ABC transporter substrate-binding protein	-
DSM110093_RS19095 (DSM110093_03889)	200378..201316	+	939	WP_243267795.1	MurR/RpiR family transcriptional regulator	-
DSM110093_RS19100 (DSM110093_03890)	201327..202649	+	1323	WP_243267796.1	glutamine synthetase family protein	-
DSM110093_RS19105 (DSM110093_03892)	202772..203038	-	267	WP_243267797.1	Txe/YoeB family addiction module toxin	Toxin
DSM110093_RS19110 (DSM110093_03893)	203035..203286	-	252	WP_243267798.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
DSM110093_RS19115 (DSM110093_03894)	203508..204857	-	1350	WP_243263448.1	Fic family protein	-
DSM110093_RS19120 (DSM110093_03895)	205498..205740	+	243	WP_243267799.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
DSM110093_RS19125 (DSM110093_03896)	205742..206053	+	312	WP_243267800.1	endoribonuclease MazF	-
DSM110093_RS19130 (DSM110093_03897)	206097..206444	-	348	WP_243267801.1	type II toxin-antitoxin system HicB family antitoxin	-
DSM110093_RS19135 (DSM110093_03898)	206441..206695	-	255	WP_243267802.1	type II toxin-antitoxin system HicA family toxin	-
DSM110093_RS19140 (DSM110093_03900)	206846..207625	-	780	WP_243267803.1	IS30 family transposase	-
DSM110093_RS19145 (DSM110093_03901)	207758..208057	-	300	WP_243268008.1	CcdB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..273772	273772

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10455.87 Da
• Isoelectric Point: 9.8911

>T220529 WP_243267797.1 NZ_CP085169:c203038-202772 [Sulfitobacter sp. DSM 110093]
VKLVFSEQAWEDYQHWVSTNDKVHERINELIKQCKRTPLKGTGKPEPLKGDLTGWWSRRISQEDRMVYRVKGRGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T220529 NZ_CP117162:1438-1761 [Sphingobium sp. AntQ-1]
GTGAACCGGGGCGACCTGGTGACGATCGCGCTGCCCGGCGATTTCGGCAAGCCGCGACCGGCTCTTATCATCCAGTCCGA
CCAGTTCGACCAGACCGGCACTGTCACGGTACTACTGGTGTCGGGTACGCTGGTGGACGCGCCCCTCATCCGCACGACGA
TCGACCCGACACCGGCCAATGGCTTGCGCAAGCGGTCGCAGGTCATGGTGGACAAGGCCATGTCGGTGAAGCGCGACAAG
ATCGGGGCGCCGATCGGCCACCTCGATGCCGAAGCCATGGTGGCGGTCACGCGAGCTCTGGCCGTCTTCTTCGCCATCGC
CTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9182.28 Da
• Isoelectric Point: 5.0481

>AT220529 WP_243267798.1 NZ_CP085169:c203286-203035 [Sulfitobacter sp. DSM 110093]
MDVMTYSDARAHLKDVMDRAINDKQEVVVTRKKSESVVVVSLDTWNAVNETLHLLSTPNNASRLRASIAQLDAGTGNERD
LAE

Nucleotide

• Length: 252 bp

>AT220529 NZ_CP117162:1217-1441 [Sphingobium sp. AntQ-1]
GTGCCGGCACCAATAACACAACGAGTCCAGAAGCGTCGCGATGCCCTGCGCGCGGCCGGTCTGCGCCCCATCCAGATATG
GGTTCCCGATACCCGACGACCGGGGTTCGCGGAAGAATGCCGGCGACAGGCGCTCGTGGTCGCGGTGGCCGACGCGGCGG
ATCATGATCTGGCCGCCTTCATGGATGCGGCCCTGATCGATCTCGACCCCGAGGGCGACGCGTGA