Detailed information of TA system TA220527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 257842..258356
• Replicon: plasmid pDSM110093_a
• Accession: NZ_CP085168
• Organism: Sulfitobacter sp. DSM 110093

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: DSM110093_RS17875
• Protein ID: WP_243267797.1
• Coordinates: 257842..258108 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: DSM110093_RS17880
• Protein ID: WP_243267798.1
• Coordinates: 258105..258356 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSM110093_RS17860 (DSM110093_03627)	254078..255223	-	1146	WP_243267794.1	ABC transporter substrate-binding protein	-
DSM110093_RS17865 (DSM110093_03628)	255448..256386	+	939	WP_243267795.1	MurR/RpiR family transcriptional regulator	-
DSM110093_RS17870 (DSM110093_03629)	256397..257719	+	1323	WP_243267796.1	glutamine synthetase family protein	-
DSM110093_RS17875 (DSM110093_03631)	257842..258108	-	267	WP_243267797.1	Txe/YoeB family addiction module toxin	Toxin
DSM110093_RS17880 (DSM110093_03632)	258105..258356	-	252	WP_243267798.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
DSM110093_RS17885 (DSM110093_03633)	258578..259927	-	1350	WP_243263448.1	Fic family protein	-
DSM110093_RS17890 (DSM110093_03634)	260568..260810	+	243	WP_243267799.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
DSM110093_RS17895 (DSM110093_03635)	260812..261123	+	312	WP_243267800.1	endoribonuclease MazF	-
DSM110093_RS17900 (DSM110093_03636)	261167..261514	-	348	WP_243267801.1	type II toxin-antitoxin system HicB family antitoxin	-
DSM110093_RS17905 (DSM110093_03637)	261511..261765	-	255	WP_243267802.1	type II toxin-antitoxin system HicA family toxin	-
DSM110093_RS17910 (DSM110093_03639)	261916..262695	-	780	WP_243267803.1	IS30 family transposase	-
DSM110093_RS17915	262904..263032	-	129	Protein_223	transposase	-
DSM110093_RS17920	263104..263337	+	234	Protein_224	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..336544	336544

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10455.87 Da
• Isoelectric Point: 9.8911

>T220527 WP_243267797.1 NZ_CP085168:c258108-257842 [Sulfitobacter sp. DSM 110093]
VKLVFSEQAWEDYQHWVSTNDKVHERINELIKQCKRTPLKGTGKPEPLKGDLTGWWSRRISQEDRMVYRVKGRGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T220527 NZ_CP117158:956646-956924 [Sphingobium sp. AntQ-1]
ATGACCTTACCGGTCGTCTGGGATGACGAGGCGGAAGCGGACCTGCTCACCATCCTCGACTATATTGGGAGGCGCAACAA
ACCGGCGGCCGATCATCTCTATGCCGCCATCCGGCATACGGCCGACAATCTGCCCAATCATCCCTATGCGCATCGCCCCG
GCCGCAGGCCCGGCACGCGCGAGGCCGTGGTCCATCCCAACTATATAATCATCTATCGCGTCGCCGCCGAAGTCATAGAG
ATATTGACGCTGGTCCATGCCCGCCAACAATATCCCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9182.28 Da
• Isoelectric Point: 5.0481

>AT220527 WP_243267798.1 NZ_CP085168:c258356-258105 [Sulfitobacter sp. DSM 110093]
MDVMTYSDARAHLKDVMDRAINDKQEVVVTRKKSESVVVVSLDTWNAVNETLHLLSTPNNASRLRASIAQLDAGTGNERD
LAE

Nucleotide

• Length: 252 bp

>AT220527 NZ_CP117158:956479-956649 [Sphingobium sp. AntQ-1]
ATGACCAAACTCACCCCCATCGAGTCCGAATTTGCGACGACCGAGGAGGCTGAAGCCCATGACGCATGGGTACAGGCCAA
GGTCGAACGTGCCCTCGCTTCCACCAACCCGCGCATCCCCCATGACGCAGTCATGGCCAAGGCGCAGGCGGTTCTCGACA
AATATCGATGA