Detailed information of TA system TA220520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 63072..63913
• Replicon: plasmid pDSM109990_a
• Accession: NZ_CP085145
• Organism: Sulfitobacter dubius strain DSM 109990

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: DSM109990_RS16555
• Protein ID: WP_243263439.1
• Coordinates: 63395..63913 (+)
• Length: 173 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: DSM109990_RS16550
• Protein ID: WP_243263438.1
• Coordinates: 63072..63398 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSM109990_RS16525 (DSM109990_03349)	58887..59102	+	216	WP_243263431.1	DUF6330 family protein	-
DSM109990_RS16530 (DSM109990_03350)	59093..59482	+	390	WP_243263433.1	DUF3768 domain-containing protein	-
DSM109990_RS16535 (DSM109990_03351)	59643..61625	+	1983	WP_243263434.1	ParB/RepB/Spo0J family partition protein	-
DSM109990_RS16540 (DSM109990_03352)	61700..62293	+	594	WP_243263435.1	regulator	-
DSM109990_RS16545 (DSM109990_03353)	62293..62946	+	654	WP_243263437.1	hypothetical protein	-
DSM109990_RS16550 (DSM109990_03354)	63072..63398	+	327	WP_243263438.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
DSM109990_RS16555 (DSM109990_03355)	63395..63913	+	519	WP_243263439.1	type II toxin-antitoxin system YhaV family toxin	Toxin
DSM109990_RS16560	64163..64315	+	153	WP_243263440.1	StaA	-
DSM109990_RS16565 (DSM109990_03356)	64418..67581	+	3164	Protein_58	strawberry notch family protein	-
DSM109990_RS16570 (DSM109990_03358)	68042..68731	+	690	WP_243263442.1	phosphate regulon transcriptional regulator PhoB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..284454	284454

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (19-155)

Antitoxin

• PrlF antitoxin (9-104)

Sequences

Toxin

Protein

• Length: 173 a.a.
• Molecular weight: 19352.95 Da
• Isoelectric Point: 9.5906

>T220520 WP_243263439.1 NZ_CP085145:63395-63913 [Sulfitobacter dubius]
MSGDSVPAQAPLVVNGWSIYAHPLFLDQLEGLIEEVEARKARDPKTWRKKNSTKRLAAIFKLVTEAIPADPGAAAFRQGG
TLGDHRKHWFRAKFFQQYRLFYRFNSDAKVIVMAWVNDDKTLRAYGSKTDAYATFKGMLEDGNPPDNFDALLKEAAAADK
RFETSLEAAPDR

Nucleotide

• Length: 519 bp

>T220520 NZ_CP117053:c4404880-4404530 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12022.54 Da
• Isoelectric Point: 4.4879

>AT220520 WP_243263438.1 NZ_CP085145:63072-63398 [Sulfitobacter dubius]
MTALAQDVSKLTDRYQTTVPAGVRKQLKLGKGDQIRYCTEPSGRVYIEPVRSDEEDPVLGAFLDFVEADIKAHPDRIRAF
DGALHDRLAALVRDIDVNLDAPLSLEDE

Nucleotide

• Length: 327 bp

>AT220520 NZ_CP117053:c4405125-4404874 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA