Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5554602..5555197 Replicon chromosome
Accession NZ_CP085135
Organism Mesorhizobium sp. PAMC28654

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag LGH82_RS27275 Protein ID WP_227345686.1
Coordinates 5554916..5555197 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag LGH82_RS27270 Protein ID WP_227345685.1
Coordinates 5554602..5554901 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGH82_RS27245 (LGH82_27245) 5549687..5551270 + 1584 WP_264484338.1 sulfotransferase -
LGH82_RS27250 (LGH82_27250) 5551518..5552003 - 486 WP_227345682.1 dihydrofolate reductase family protein -
LGH82_RS27255 (LGH82_27255) 5552098..5552451 - 354 WP_227345683.1 DUF1428 family protein -
LGH82_RS27260 (LGH82_27260) 5552635..5553057 - 423 WP_227345684.1 glyoxalase/bleomycin resistance/extradiol dioxygenase family protein -
LGH82_RS27265 (LGH82_27265) 5553917..5553918 - 2 WP_227343924.1 IS630 family transposase -
LGH82_RS27270 (LGH82_27270) 5554602..5554901 - 300 WP_227345685.1 HigA family addiction module antitoxin Antitoxin
LGH82_RS27275 (LGH82_27275) 5554916..5555197 - 282 WP_227345686.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LGH82_RS27280 (LGH82_27280) 5555480..5556630 + 1151 WP_227343807.1 IS3 family transposase -
LGH82_RS27285 (LGH82_27285) 5556593..5558101 - 1509 WP_227345687.1 ABC-F family ATP-binding cassette domain-containing protein -
LGH82_RS27290 (LGH82_27290) 5558395..5558934 - 540 WP_227345688.1 hypothetical protein -
LGH82_RS27295 (LGH82_27295) 5558946..5559845 - 900 WP_227345689.1 DMT family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 5553312..5556630 3318


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(8-79)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10531.89 Da        Isoelectric Point: 9.0992

>T220511 WP_227345686.1 NZ_CP085135:c5555197-5554916 [Mesorhizobium sp. PAMC28654]
MIRSFKDKTSEAVSNGKAPKGFPSDLVRSAQRKLFMIDNAAELSDLKTPPGNRLEPLKGNRAGQHSIRINDQFRICFRWV
DGQAEDVEITDYH

Download         Length: 282 bp

>T220511 NZ_CP117053:2754903-2755010 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11137.95 Da        Isoelectric Point: 6.4670

>AT220511 WP_227345685.1 NZ_CP085135:c5554901-5554602 [Mesorhizobium sp. PAMC28654]
MLNINPLIHPGEILREEYLIPLGLKPYTLAKKLHVPRTRIERLASETTAVTPDTALRLAKFFGTTPQFWMNMQASYDLAV
EGEVKKADLETIESLQTAA

Download         Length: 300 bp

>AT220511 NZ_CP117053:c2754856-2754790 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References