Detailed information of TA system TA220504

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1471604..1472250
• Replicon: chromosome
• Accession: NZ_CP085096
• Organism: Aggregatibacter actinomycetemcomitans strain 4S

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X2JQ23
• Locus tag: LIN98_RS07375
• Protein ID: WP_005555126.1
• Coordinates: 1471900..1472250 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: X2JT85
• Locus tag: LIN98_RS07370
• Protein ID: WP_005540827.1
• Coordinates: 1471604..1471903 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN98_RS07360	1468206..1468433	-	228	WP_005567423.1	hypothetical protein	-
LIN98_RS07365	1468635..1471568	+	2934	WP_014167733.1	bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase	-
LIN98_RS07370	1471604..1471903	-	300	WP_005540827.1	helix-turn-helix transcriptional regulator	Antitoxin
LIN98_RS07375	1471900..1472250	-	351	WP_005555126.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LIN98_RS07380	1472438..1474021	-	1584	WP_005567417.1	peptide chain release factor 3	-
LIN98_RS07385	1474378..1475205	+	828	WP_005540818.1	hypothetical protein	-
LIN98_RS07390	1475300..1476637	+	1338	WP_005567415.1	argininosuccinate synthase	-
LIN98_RS07395	1476827..1477072	+	246	WP_005540815.1	YgjV family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13518.64 Da
• Isoelectric Point: 9.9664

>T220504 WP_005555126.1 NZ_CP085096:c1472250-1471900 [Aggregatibacter actinomycetemcomitans]
MWAVILTERFETWLNQQPIKTQECVLANLVRLEHFGPNLARPYVDSVAGSIHSNMKELRVQHSGRAIRLFFAFDPKRRAI
VLCGGDKGNDKRFYRTMIRIADQELNHYLATMEQTK

Nucleotide

• Length: 351 bp

>T220504 NZ_CP117053:1066175-1066486 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTATACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11342.12 Da
• Isoelectric Point: 5.8598

>AT220504 WP_005540827.1 NZ_CP085096:c1471903-1471604 [Aggregatibacter actinomycetemcomitans]
MKTLQQVIDERSVESRQRIQTMAEDLILETGLQLLREELNISQQQLATILGVTQPAINQLEQRGNEIKLATLKRYIEAMG
GKLRLSVEMPDGHQRVFQI

Nucleotide

• Length: 300 bp

>AT220504 NZ_CP117053:1066483-1066902 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCCGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTACGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGTGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: X2JQ23

Antitoxin

• AlphaFold DB: X2JT85