Detailed information of TA system TA220501

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1224044..1224610
• Replicon: chromosome
• Accession: NZ_CP085096
• Organism: Aggregatibacter actinomycetemcomitans strain 4S

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X2JU35
• Locus tag: LIN98_RS06260
• Protein ID: WP_005543444.1
• Coordinates: 1224332..1224610 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: LIN98_RS06255
• Protein ID: WP_005543446.1
• Coordinates: 1224044..1224316 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN98_RS06230	1220583..1220849	-	267	WP_005543457.1	oxaloacetate decarboxylase subunit gamma	-
LIN98_RS06235	1221211..1221402	+	192	WP_005553960.1	hypothetical protein	-
LIN98_RS06240	1221377..1221754	+	378	WP_005543455.1	hypothetical protein	-
LIN98_RS06245	1221875..1222150	-	276	WP_005561117.1	hydrogenase maturation factor HybG	-
LIN98_RS06250	1222605..1224035	-	1431	WP_005566276.1	bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF	-
LIN98_RS06255	1224044..1224316	-	273	WP_005543446.1	HigA family addiction module antitoxin	Antitoxin
LIN98_RS06260	1224332..1224610	-	279	WP_005543444.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LIN98_RS06265	1224705..1225706	-	1002	WP_014167798.1	anthranilate phosphoribosyltransferase	-
LIN98_RS06270	1225718..1226110	-	393	WP_005543439.1	tautomerase family protein	-
LIN98_RS06275	1226184..1226774	-	591	WP_005572120.1	aminodeoxychorismate/anthranilate synthase component II	-
LIN98_RS06280	1226785..1228332	-	1548	WP_005572118.1	anthranilate synthase component 1	-
LIN98_RS06285	1228500..1229054	-	555	WP_005543432.1	HAD family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1160833..1338366	177533

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-79)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 9.5983

>T220501 WP_005543444.1 NZ_CP085096:c1224610-1224332 [Aggregatibacter actinomycetemcomitans]
MILSFKHKGLEQFFKTGSTAGIQAKHAKKLNLQLATLNNAETPLAMSVPSWNLHKLKGDLQEHWAVTVNGNWRLTFKFEN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T220501 NZ_CP117053:726960-727256 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10138.74 Da
• Isoelectric Point: 7.3169

>AT220501 WP_005543446.1 NZ_CP085096:c1224316-1224044 [Aggregatibacter actinomycetemcomitans]
MHNPPHPGELLKEYIDGVSVTKVAQKLGVSRVTLSRILNGKASITPEMAVRLSELLNTTTPKLWLGMQADYDLWQIEQQH
AVFNIQPLFN

Nucleotide

• Length: 273 bp

>AT220501 NZ_CP117053:727258-727653 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: X2JU35