Detailed information of TA system TA220488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1527207..1527773
• Replicon: chromosome
• Accession: NZ_CP085094
• Organism: Aggregatibacter actinomycetemcomitans strain 5S

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X2JU35
• Locus tag: LIN95_RS07760
• Protein ID: WP_005543444.1
• Coordinates: 1527207..1527485 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: LIN95_RS07765
• Protein ID: WP_005567380.1
• Coordinates: 1527501..1527773 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN95_RS07735	1522763..1523317	+	555	WP_005543432.1	HAD family hydrolase	-
LIN95_RS07740	1523485..1525032	+	1548	WP_025298400.1	anthranilate synthase component 1	-
LIN95_RS07745	1525043..1525633	+	591	WP_005543437.1	aminodeoxychorismate/anthranilate synthase component II	-
LIN95_RS07750	1525707..1526099	+	393	WP_005543439.1	tautomerase family protein	-
LIN95_RS07755	1526111..1527112	+	1002	WP_233115388.1	anthranilate phosphoribosyltransferase	-
LIN95_RS07760	1527207..1527485	+	279	WP_005543444.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LIN95_RS07765	1527501..1527773	+	273	WP_005567380.1	HigA family addiction module antitoxin	Antitoxin
LIN95_RS07770	1527782..1529212	+	1431	WP_005566276.1	bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF	-
LIN95_RS07775	1529667..1529942	+	276	WP_005561117.1	hydrogenase maturation factor HybG	-
LIN95_RS07780	1530063..1530440	-	378	WP_005543455.1	hypothetical protein	-
LIN95_RS07785	1530415..1530606	-	192	WP_005553960.1	hypothetical protein	-
LIN95_RS07790	1530967..1531233	+	267	WP_005543457.1	oxaloacetate decarboxylase subunit gamma	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (2-87)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 9.5983

>T220488 WP_005543444.1 NZ_CP085094:1527207-1527485 [Aggregatibacter actinomycetemcomitans]
MILSFKHKGLEQFFKTGSTAGIQAKHAKKLNLQLATLNNAETPLAMSVPSWNLHKLKGDLQEHWAVTVNGNWRLTFKFEN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T220488 NZ_CP117049:3571419-3571637 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10164.78 Da
• Isoelectric Point: 7.1995

>AT220488 WP_005567380.1 NZ_CP085094:1527501-1527773 [Aggregatibacter actinomycetemcomitans]
MHNPPYPGELLKEYIDGVSVTKVAQKLGVSRVTLSRILNGKASITPEMAVRLSELLNTTTPKLWLGMQADYDLWQIEQQH
AVFNIQPLFN

Nucleotide

• Length: 273 bp

>AT220488 NZ_CP117049:3571019-3571393 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: X2JU35