Detailed information of TA system TA220479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1541887..1542453
• Replicon: chromosome
• Accession: NZ_CP085093
• Organism: Aggregatibacter actinomycetemcomitans strain 23S

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X2JU35
• Locus tag: LIN99_RS07880
• Protein ID: WP_005543444.1
• Coordinates: 1541887..1542165 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: LIN99_RS07885
• Protein ID: WP_005567380.1
• Coordinates: 1542181..1542453 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN99_RS07855	1537443..1537997	+	555	WP_005543432.1	HAD family hydrolase	-
LIN99_RS07860	1538165..1539712	+	1548	WP_025298400.1	anthranilate synthase component 1	-
LIN99_RS07865	1539723..1540313	+	591	WP_005543437.1	aminodeoxychorismate/anthranilate synthase component II	-
LIN99_RS07870	1540387..1540779	+	393	WP_005543439.1	tautomerase family protein	-
LIN99_RS07875	1540791..1541792	+	1002	WP_233115388.1	anthranilate phosphoribosyltransferase	-
LIN99_RS07880	1541887..1542165	+	279	WP_005543444.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LIN99_RS07885	1542181..1542453	+	273	WP_005567380.1	HigA family addiction module antitoxin	Antitoxin
LIN99_RS07890	1542462..1543892	+	1431	WP_005566276.1	bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF	-
LIN99_RS07895	1544347..1544622	+	276	WP_005561117.1	hydrogenase maturation factor HybG	-
LIN99_RS07900	1544743..1545120	-	378	WP_005543455.1	hypothetical protein	-
LIN99_RS07905	1545095..1545286	-	192	WP_005553960.1	hypothetical protein	-
LIN99_RS07910	1545647..1545913	+	267	WP_005543457.1	oxaloacetate decarboxylase subunit gamma	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (2-87)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 9.5983

>T220479 WP_005543444.1 NZ_CP085093:1541887-1542165 [Aggregatibacter actinomycetemcomitans]
MILSFKHKGLEQFFKTGSTAGIQAKHAKKLNLQLATLNNAETPLAMSVPSWNLHKLKGDLQEHWAVTVNGNWRLTFKFEN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T220479 NZ_CP117049:1819753-1820004 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10164.78 Da
• Isoelectric Point: 7.1995

>AT220479 WP_005567380.1 NZ_CP085093:1542181-1542453 [Aggregatibacter actinomycetemcomitans]
MHNPPYPGELLKEYIDGVSVTKVAQKLGVSRVTLSRILNGKASITPEMAVRLSELLNTTTPKLWLGMQADYDLWQIEQQH
AVFNIQPLFN

Nucleotide

• Length: 273 bp

>AT220479 NZ_CP117049:1820001-1820255 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: X2JU35