Detailed information of TA system TA220476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1276313..1276959
• Replicon: chromosome
• Accession: NZ_CP085093
• Organism: Aggregatibacter actinomycetemcomitans strain 23S

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X2JQ23
• Locus tag: LIN99_RS06610
• Protein ID: WP_005555126.1
• Coordinates: 1276313..1276663 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: X2JT85
• Locus tag: LIN99_RS06615
• Protein ID: WP_005540827.1
• Coordinates: 1276660..1276959 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN99_RS06590	1271491..1271736	-	246	WP_005540815.1	YgjV family protein	-
LIN99_RS06595	1271926..1273263	-	1338	WP_005567415.1	argininosuccinate synthase	-
LIN99_RS06600	1273358..1274185	-	828	WP_233115378.1	SDR family NAD(P)-dependent oxidoreductase	-
LIN99_RS06605	1274542..1276125	+	1584	WP_005567417.1	peptide chain release factor 3	-
LIN99_RS06610	1276313..1276663	+	351	WP_005555126.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LIN99_RS06615	1276660..1276959	+	300	WP_005540827.1	helix-turn-helix transcriptional regulator	Antitoxin
LIN99_RS06620	1276995..1279928	-	2934	WP_005567421.1	bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase	-
LIN99_RS06625	1280130..1280357	+	228	WP_005567423.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13518.64 Da
• Isoelectric Point: 9.9664

>T220476 WP_005555126.1 NZ_CP085093:1276313-1276663 [Aggregatibacter actinomycetemcomitans]
MWAVILTERFETWLNQQPIKTQECVLANLVRLEHFGPNLARPYVDSVAGSIHSNMKELRVQHSGRAIRLFFAFDPKRRAI
VLCGGDKGNDKRFYRTMIRIADQELNHYLATMEQTK

Nucleotide

• Length: 351 bp

>T220476 NZ_CP117049:834484-834891 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11342.12 Da
• Isoelectric Point: 5.8598

>AT220476 WP_005540827.1 NZ_CP085093:1276660-1276959 [Aggregatibacter actinomycetemcomitans]
MKTLQQVIDERSVESRQRIQTMAEDLILETGLQLLREELNISQQQLATILGVTQPAINQLEQRGNEIKLATLKRYIEAMG
GKLRLSVEMPDGHQRVFQI

Nucleotide

• Length: 300 bp

>AT220476 NZ_CP117049:834237-834503 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCCGATC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: X2JQ23

Antitoxin

• AlphaFold DB: X2JT85