Detailed information of TA system TA220468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1260886..1261532
• Replicon: chromosome
• Accession: NZ_CP085092
• Organism: Aggregatibacter actinomycetemcomitans strain 30R

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X2JQ23
• Locus tag: LIN94_RS06415
• Protein ID: WP_005555126.1
• Coordinates: 1260886..1261236 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: X2JT85
• Locus tag: LIN94_RS06420
• Protein ID: WP_005540827.1
• Coordinates: 1261233..1261532 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN94_RS06395	1256064..1256309	-	246	WP_005540815.1	YgjV family protein	-
LIN94_RS06400	1256499..1257836	-	1338	WP_005567415.1	argininosuccinate synthase	-
LIN94_RS06405	1257931..1258758	-	828	WP_005540818.1	hypothetical protein	-
LIN94_RS06410	1259115..1260698	+	1584	WP_005567417.1	peptide chain release factor 3	-
LIN94_RS06415	1260886..1261236	+	351	WP_005555126.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LIN94_RS06420	1261233..1261532	+	300	WP_005540827.1	helix-turn-helix transcriptional regulator	Antitoxin
LIN94_RS06425	1261568..1264501	-	2934	WP_014167733.1	bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase	-
LIN94_RS06430	1264703..1264930	+	228	WP_005567423.1	hypothetical protein	-
LIN94_RS06435	1264923..1265684	+	762	WP_025298334.1	CRISPR-associated endonuclease Cas3''	-
LIN94_RS06440	1266030..1266430	+	401	Protein_1243	bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13518.64 Da
• Isoelectric Point: 9.9664

>T220468 WP_005555126.1 NZ_CP085092:1260886-1261236 [Aggregatibacter actinomycetemcomitans]
MWAVILTERFETWLNQQPIKTQECVLANLVRLEHFGPNLARPYVDSVAGSIHSNMKELRVQHSGRAIRLFFAFDPKRRAI
VLCGGDKGNDKRFYRTMIRIADQELNHYLATMEQTK

Nucleotide

• Length: 351 bp

>T220468 NZ_CP117039:1323832-1324119 [Vibrio parahaemolyticus]
ATGAAAGTAGTTTGGTCTCCACTAGCGCTTCAGAAGCTAGGTGATGCAGCGGAATTTATTTCTTTGGATAATCCAGCTGC
AGCAGAAAACTGGATAAACGAAGTTTTTGACAAAACTGAACTACTTGGAAGTATGCCTGAAATGGGGCGCTTTGTTCCTG
AAATGCCCAATACTAACTATCGTGAAATCATTTTTGGTCACTATCGTATTATCTATAGCTTAAGCCATGAAATCCGAGTA
CTGACAGTTCGTAATTGCCGTCAAATGTTGTCGGAAGATGACCTGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11342.12 Da
• Isoelectric Point: 5.8598

>AT220468 WP_005540827.1 NZ_CP085092:1261233-1261532 [Aggregatibacter actinomycetemcomitans]
MKTLQQVIDERSVESRQRIQTMAEDLILETGLQLLREELNISQQQLATILGVTQPAINQLEQRGNEIKLATLKRYIEAMG
GKLRLSVEMPDGHQRVFQI

Nucleotide

• Length: 300 bp

>AT220468 NZ_CP117039:1323554-1323835 [Vibrio parahaemolyticus]
ATGAGCCGTATTCATTTCGATCAAGATATTCAGCCGTTATCTGAATTTCGCGCAGGTGTAGCTTCTTACATCAAACAAAT
CAACGAGACTCGCCGTCCATTGGTTATCACACAGCGTGGTAAAGGTGTAGCTGTTGTTCTCGATGTTGCAGAGTATGAAG
CAATGCAAGAGAAGATCGAGTTACTGGAAGAAATGCGCACAGCTGAAGCTCAATTAGCTTCAGGCCTTGGTATTTCAAAT
GATGATGCTCGTTCTCAAGTACTGGGGCGCATTAAGAAATGA

Structures

Toxin

• AlphaFold DB: X2JQ23

Antitoxin

• AlphaFold DB: X2JT85