Detailed information of TA system TA220452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1250984..1251630
• Replicon: chromosome
• Accession: NZ_CP085090
• Organism: Aggregatibacter actinomycetemcomitans strain 31R

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X2JQ23
• Locus tag: LIN96_RS06355
• Protein ID: WP_005555126.1
• Coordinates: 1250984..1251334 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: X2JT85
• Locus tag: LIN96_RS06360
• Protein ID: WP_005540827.1
• Coordinates: 1251331..1251630 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN96_RS06335	1246162..1246407	-	246	WP_005540815.1	YgjV family protein	-
LIN96_RS06340	1246597..1247934	-	1338	WP_249847408.1	argininosuccinate synthase	-
LIN96_RS06345	1248029..1248856	-	828	WP_005540818.1	hypothetical protein	-
LIN96_RS06350	1249213..1250796	+	1584	WP_249847410.1	peptide chain release factor 3	-
LIN96_RS06355	1250984..1251334	+	351	WP_005555126.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LIN96_RS06360	1251331..1251630	+	300	WP_005540827.1	helix-turn-helix transcriptional regulator	Antitoxin
LIN96_RS06365	1251666..1254599	-	2934	WP_249847411.1	bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase	-
LIN96_RS06370	1254801..1255028	+	228	WP_005567423.1	hypothetical protein	-
LIN96_RS06375	1255021..1255782	+	762	WP_025298334.1	CRISPR-associated endonuclease Cas3''	-
LIN96_RS06380	1256128..1256528	+	401	Protein_1232	bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13518.64 Da
• Isoelectric Point: 9.9664

>T220452 WP_005555126.1 NZ_CP085090:1250984-1251334 [Aggregatibacter actinomycetemcomitans]
MWAVILTERFETWLNQQPIKTQECVLANLVRLEHFGPNLARPYVDSVAGSIHSNMKELRVQHSGRAIRLFFAFDPKRRAI
VLCGGDKGNDKRFYRTMIRIADQELNHYLATMEQTK

Nucleotide

• Length: 351 bp

>T220452 NZ_CP117033:881104-881502 [Salmonella enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11342.12 Da
• Isoelectric Point: 5.8598

>AT220452 WP_005540827.1 NZ_CP085090:1251331-1251630 [Aggregatibacter actinomycetemcomitans]
MKTLQQVIDERSVESRQRIQTMAEDLILETGLQLLREELNISQQQLATILGVTQPAINQLEQRGNEIKLATLKRYIEAMG
GKLRLSVEMPDGHQRVFQI

Nucleotide

• Length: 300 bp

>AT220452 NZ_CP117033:880877-881104 [Salmonella enterica]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Toxin

• AlphaFold DB: X2JQ23

Antitoxin

• AlphaFold DB: X2JT85