Detailed information of TA system TA220446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1337840..1338406
• Replicon: chromosome
• Accession: NZ_CP085089
• Organism: Aggregatibacter actinomycetemcomitans strain 31S

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X2JU35
• Locus tag: LIN92_RS06670
• Protein ID: WP_005543444.1
• Coordinates: 1337840..1338118 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: LIN92_RS06675
• Protein ID: WP_005543446.1
• Coordinates: 1338134..1338406 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN92_RS06645	1333396..1333950	+	555	WP_005543432.1	HAD family hydrolase	-
LIN92_RS06650	1334118..1335665	+	1548	WP_249847443.1	anthranilate synthase component 1	-
LIN92_RS06655	1335676..1336266	+	591	WP_005543437.1	aminodeoxychorismate/anthranilate synthase component II	-
LIN92_RS06660	1336340..1336732	+	393	WP_005543439.1	tautomerase family protein	-
LIN92_RS06665	1336744..1337745	+	1002	WP_005567382.1	anthranilate phosphoribosyltransferase	-
LIN92_RS06670	1337840..1338118	+	279	WP_005543444.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LIN92_RS06675	1338134..1338406	+	273	WP_005543446.1	HigA family addiction module antitoxin	Antitoxin
LIN92_RS06680	1338415..1339841	+	1427	Protein_1287	bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF	-
LIN92_RS06685	1340296..1340571	+	276	WP_005561117.1	hydrogenase maturation factor HybG	-
LIN92_RS06690	1340692..1341069	-	378	WP_005543455.1	hypothetical protein	-
LIN92_RS06695	1341044..1341235	-	192	WP_005553960.1	hypothetical protein	-
LIN92_RS06700	1341596..1341862	+	267	WP_249847445.1	oxaloacetate decarboxylase subunit gamma	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-79)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 9.5983

>T220446 WP_005543444.1 NZ_CP085089:1337840-1338118 [Aggregatibacter actinomycetemcomitans]
MILSFKHKGLEQFFKTGSTAGIQAKHAKKLNLQLATLNNAETPLAMSVPSWNLHKLKGDLQEHWAVTVNGNWRLTFKFEN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T220446 NZ_CP117029:c1637688-1637434 [Vibrio tubiashii]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATGGTCTTAAAGCTGTTCTAGACGGTGTAGTTAATGACGCAGATACAAC
AGTCATTACACGCCGTGACTCTGAAGATGCTGTGGTTATGTCTTTAGATTACTACAATAGCTTAATGGAAACAGTGCATT
TACTACGCTCTCCTCAGAATGCTGAACACCTAAACCGCTCCATAGCACAGTATCGTGCTGGTCAAACAACTGAGCGAGAT
TTAATTGATGAGTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10138.74 Da
• Isoelectric Point: 7.3169

>AT220446 WP_005543446.1 NZ_CP085089:1338134-1338406 [Aggregatibacter actinomycetemcomitans]
MHNPPHPGELLKEYIDGVSVTKVAQKLGVSRVTLSRILNGKASITPEMAVRLSELLNTTTPKLWLGMQADYDLWQIEQQH
AVFNIQPLFN

Nucleotide

• Length: 273 bp

>AT220446 NZ_CP117029:c1637441-1637172 [Vibrio tubiashii]
ATGAGTAGCAGTCTACGTTTACTATCGTGGACTGACGACGCTTGGGGTGACTACCTGTATTGGCAAACGCAAGACAAAAA
AACGCTCAAGCGCATCAACAAACTCATCAATGATGTTAAGCGTTCTCCTTTTGAGGGCATTGGTAAACCTGAACCGTTAA
AAGAGAACTTATCTGGTTTTTGGTCTCGTCGTATTGATGATACCAACAGGCTTGTTTATGCAGTCGATGATCAAGCGATA
ACCATTATTTCGTGTCGTTACCACTACTAA

Structures

Toxin

• AlphaFold DB: X2JU35