Detailed information of TA system TA220444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1093227..1093873
• Replicon: chromosome
• Accession: NZ_CP085089
• Organism: Aggregatibacter actinomycetemcomitans strain 31S

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X2JQ23
• Locus tag: LIN92_RS05555
• Protein ID: WP_005555126.1
• Coordinates: 1093227..1093577 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: X2JT85
• Locus tag: LIN92_RS05560
• Protein ID: WP_005540827.1
• Coordinates: 1093574..1093873 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN92_RS05535	1088405..1088650	-	246	WP_005540815.1	YgjV family protein	-
LIN92_RS05540	1088840..1090177	-	1338	WP_249847408.1	argininosuccinate synthase	-
LIN92_RS05545	1090272..1091099	-	828	WP_005540818.1	hypothetical protein	-
LIN92_RS05550	1091456..1093039	+	1584	WP_249847410.1	peptide chain release factor 3	-
LIN92_RS05555	1093227..1093577	+	351	WP_005555126.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LIN92_RS05560	1093574..1093873	+	300	WP_005540827.1	helix-turn-helix transcriptional regulator	Antitoxin
LIN92_RS05565	1093909..1096842	-	2934	WP_249847411.1	bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase	-
LIN92_RS05570	1097044..1097271	+	228	WP_005567423.1	hypothetical protein	-
LIN92_RS05575	1097264..1098025	+	762	WP_025298334.1	CRISPR-associated endonuclease Cas3''	-
LIN92_RS05580	1098371..1098771	+	401	Protein_1072	bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13518.64 Da
• Isoelectric Point: 9.9664

>T220444 WP_005555126.1 NZ_CP085089:1093227-1093577 [Aggregatibacter actinomycetemcomitans]
MWAVILTERFETWLNQQPIKTQECVLANLVRLEHFGPNLARPYVDSVAGSIHSNMKELRVQHSGRAIRLFFAFDPKRRAI
VLCGGDKGNDKRFYRTMIRIADQELNHYLATMEQTK

Nucleotide

• Length: 351 bp

>T220444 NZ_CP117025:3050738-3051067 [Sphingomonas hankookensis]
ATGGCTAAGGGCGCTCAGACCAAGCGCGCCTCTCATCCCCGTGCCTGCGACTATACGAAGGCGTTCAGCAAGGATTGGGC
ACGCCTGTCCGGTTCGGGCCGGTATGACATGAACCGGCTGAAGGCCGTCATGCTCCTGCTCATCGCCAACGACGCGCCGC
TGGGGCCGGAGTGGAAGGATCACCCGCTCAAGGGCGAATGGTCCTCGCACCGCGAATGCCATGTCGGCGGGGACTTTCTG
CTCATCTACAAAACAGACGACGGTGCCGGTAAGGGCGGCACCATCGTCTTCGTCAGGGCTGGAACGCACGCCGACCTGTT
CGGCGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11342.12 Da
• Isoelectric Point: 5.8598

>AT220444 WP_005540827.1 NZ_CP085089:1093574-1093873 [Aggregatibacter actinomycetemcomitans]
MKTLQQVIDERSVESRQRIQTMAEDLILETGLQLLREELNISQQQLATILGVTQPAINQLEQRGNEIKLATLKRYIEAMG
GKLRLSVEMPDGHQRVFQI

Nucleotide

• Length: 300 bp

>AT220444 NZ_CP117025:3050482-3050745 [Sphingomonas hankookensis]
ATGGCCTCTACCATGCTTCACGTCCGGCTGGACGAAGATATCAAGACCAAGGGCAATGCAGCGCTCGCTGCGATGGGCCT
GTCGGCGGCGGATGCGGTGCGGCTTCTGTATCACCGCATCGTCGCCGATCAGGCGCTCCCGCTCGAACTGAAGGTACCCA
ACGCCGCCACTCGGCAAGCGATGCTCGAAGCGGATGCCATCATTGCCTCGCGTGCGGACCGGTTCGAGAATGGTGAGGCG
ATGATCGGCACGCTGGATGGCTAA

Structures

Toxin

• AlphaFold DB: X2JQ23

Antitoxin

• AlphaFold DB: X2JT85