Detailed information of TA system TA220415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5242073..5242668
• Replicon: chromosome
• Accession: NZ_CP085082
• Organism: Pseudomonas aeruginosa strain PAO1-Holloway

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: LIO20_RS24270
• Protein ID: WP_003113526.1
• Coordinates: 5242390..5242668 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LIO20_RS24265
• Protein ID: WP_003113527.1
• Coordinates: 5242073..5242378 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIO20_RS24230 (LIO20_24190)	5237212..5238060	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
LIO20_RS24240 (LIO20_24200)	5238227..5239168	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
LIO20_RS24245 (LIO20_24205)	5239285..5239899	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
LIO20_RS24250 (LIO20_24210)	5239941..5240525	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
LIO20_RS24255 (LIO20_24215)	5240566..5241666	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
LIO20_RS24265 (LIO20_24225)	5242073..5242378	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
LIO20_RS24270 (LIO20_24230)	5242390..5242668	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LIO20_RS24275 (LIO20_24235)	5242721..5242849	-	129	Protein_4793	integrase	-
LIO20_RS24280 (LIO20_24240)	5242997..5245225	+	2229	WP_003113525.1	TonB-dependent receptor	-
LIO20_RS24285 (LIO20_24245)	5245295..5245942	-	648	WP_003095021.1	carbonate dehydratase	-
LIO20_RS24290 (LIO20_24250)	5246004..5247242	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T220415 WP_003113526.1 NZ_CP085082:c5242668-5242390 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T220415 NZ_CP117016:5016320-5016694 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT220415 WP_003113527.1 NZ_CP085082:c5242378-5242073 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT220415 NZ_CP117016:5015899-5016273 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: V6ALY3