Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1229720..1230369 Replicon chromosome
Accession NZ_CP085080
Organism Enterobacter cloacae strain ECl_1.VN

Toxin (Protein)


Gene name higB Uniprot ID A0A5E1A383
Locus tag LH408_RS05740 Protein ID WP_013097692.1
Coordinates 1230007..1230369 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A5E1A3P3
Locus tag LH408_RS05735 Protein ID WP_013097693.1
Coordinates 1229720..1230019 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH408_RS05725 (LH408_05725) 1225638..1227611 + 1974 WP_045902386.1 PhoX family phosphatase -
LH408_RS05730 (LH408_05730) 1227714..1229102 + 1389 WP_063425185.1 phenylalanine transporter -
LH408_RS05735 (LH408_05735) 1229720..1230019 - 300 WP_013097693.1 helix-turn-helix transcriptional regulator Antitoxin
LH408_RS05740 (LH408_05740) 1230007..1230369 - 363 WP_013097692.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LH408_RS05745 (LH408_05745) 1230575..1231813 + 1239 WP_226835832.1 MFS transporter -
LH408_RS05750 (LH408_05750) 1231829..1232854 + 1026 WP_226835834.1 sugar phosphate isomerase/epimerase -
LH408_RS05755 (LH408_05755) 1232847..1233989 + 1143 WP_226835836.1 Gfo/Idh/MocA family oxidoreductase -
LH408_RS05760 (LH408_05760) 1234065..1235036 + 972 WP_023620419.1 LacI family DNA-binding transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13994.00 Da        Isoelectric Point: 7.3235

>T220396 WP_013097692.1 NZ_CP085080:c1230369-1230007 [Enterobacter cloacae]
MWNVETTDRFDEWFFEQTTALKEDVLAAMHILSEFGPQLGRPFVDTVKASAFGNMKELRIQHRGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYKEMIALADVEYHRHLANKEAIWQR

Download         Length: 363 bp

>T220396 NZ_CP117013:c4696919-4696569 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10875.62 Da        Isoelectric Point: 8.6152

>AT220396 WP_013097693.1 NZ_CP085080:c1230019-1229720 [Enterobacter cloacae]
MATLKELMAQQSAESQERIAAKVEVMRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GELSIDVTLPNGKRIAFEI

Download         Length: 300 bp

>AT220396 NZ_CP117013:c4697164-4696913 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A383

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1A3P3

References