Detailed information of TA system TA220382

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 91717..92138
• Replicon: plasmid pNDM_P21_SE1_04.20
• Accession: NZ_CP085075
• Organism: Escherichia coli strain P21_SE1_04.20

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LH372_RS24580
• Protein ID: WP_001375150.1
• Coordinates: 92022..92138 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	91717..91915 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH372_RS24545 (89053)	89053..89157	-	105	Protein_100	thiosulfate reductase cytochrome B subunit	-
LH372_RS24550 (89214)	89214..89907	+	694	Protein_101	IS1 family transposase	-
LH372_RS24555 (90034)	90034..90546	+	513	WP_223350513.1	potassium channel family protein	-
LH372_RS24560 (90631)	90631..90882	+	252	Protein_103	iron-containing alcohol dehydrogenase	-
LH372_RS24565 (91171)	91171..91488	-	318	Protein_104	IS110 family transposase	-
LH372_RS24570 (91643)	91643..91748	+	106	Protein_105	plasmid SOS inhibition protein A	-
- (91717)	91717..91915	+	199	NuclAT_0	-	Antitoxin
- (91717)	91717..91915	+	199	NuclAT_0	-	Antitoxin
- (91717)	91717..91915	+	199	NuclAT_0	-	Antitoxin
- (91717)	91717..91915	+	199	NuclAT_0	-	Antitoxin
LH372_RS24575 (91922)	91922..92071	+	150	Protein_106	DUF5431 family protein	-
LH372_RS24580 (92022)	92022..92138	+	117	WP_001375150.1	type I toxin-antitoxin system Hok family toxin	Toxin
LH372_RS24585 (92439)	92439..92736	-	298	Protein_108	hypothetical protein	-
LH372_RS24590 (92806)	92806..93012	+	207	WP_000275854.1	hypothetical protein	-
LH372_RS24595 (93037)	93037..93324	+	288	WP_000107242.1	hypothetical protein	-
LH372_RS24600 (93443)	93443..94264	+	822	WP_001234465.1	DUF932 domain-containing protein	-
LH372_RS24605 (94560)	94560..95132	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
LH372_RS24610 (95502)	95502..95885	+	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LH372_RS24615 (96076)	96076..96474	+	399	WP_226907400.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / blaCTX-M-15 / mph(A) / aadA5	-	1..134984	134984
	-	inside	IScluster/Tn	-	-	87982..96862	8880

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4481.27 Da
• Isoelectric Point: 8.4890

>T220382 WP_001375150.1 NZ_CP085075:92022-92138 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T220382 NZ_CP117013:1190943-1191278 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAAAGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT220382 NZ_CP085075:91717-91915 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin