Detailed information of TA system TA220375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3839004..3839698
• Replicon: chromosome
• Accession: NZ_CP085074
• Organism: Escherichia coli strain P21_SE1_04.20

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: LH372_RS18610
• Protein ID: WP_001263489.1
• Coordinates: 3839004..3839402 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: LH372_RS18615
• Protein ID: WP_000554758.1
• Coordinates: 3839405..3839698 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3834592)	3834592..3834672	-	81	NuclAT_13	-	-
- (3834592)	3834592..3834672	-	81	NuclAT_13	-	-
- (3834592)	3834592..3834672	-	81	NuclAT_13	-	-
- (3834592)	3834592..3834672	-	81	NuclAT_13	-	-
LH372_RS18585 (3835268)	3835268..3835726	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LH372_RS18590 (3835987)	3835987..3837444	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
LH372_RS18595 (3837501)	3837501..3838022	-	522	Protein_3642	peptide chain release factor H	-
LH372_RS18600 (3838018)	3838018..3838224	-	207	Protein_3643	RtcB family protein	-
LH372_RS18605 (3838542)	3838542..3838994	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
LH372_RS18610 (3839004)	3839004..3839402	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LH372_RS18615 (3839405)	3839405..3839698	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LH372_RS18620 (3839750)	3839750..3840805	-	1056	WP_001226164.1	DNA polymerase IV	-
LH372_RS18625 (3840876)	3840876..3841661	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
LH372_RS18630 (3841633)	3841633..3843345	+	1713	Protein_3649	flagellar biosynthesis protein FlhA	-
LH372_RS18635 (3843569)	3843569..3844066	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3820440..3844868	24428

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T220375 WP_001263489.1 NZ_CP085074:c3839402-3839004 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T220375 NZ_CP117010:3432173-3432391 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT220375 WP_000554758.1 NZ_CP085074:c3839698-3839405 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT220375 NZ_CP117010:3431773-3432147 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3