Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yefM-yoeB (relBE)/YoeB-YefM |
| Location | 1954140..1954642 | Replicon | chromosome |
| Accession | NZ_CP085074 | ||
| Organism | Escherichia coli strain P21_SE1_04.20 | ||
Toxin (Protein)
| Gene name | yoeB | Uniprot ID | U9YDH0 |
| Locus tag | LH372_RS09410 | Protein ID | WP_000767829.1 |
| Coordinates | 1954388..1954642 (+) | Length | 85 a.a. |
Antitoxin (Protein)
| Gene name | yefM | Uniprot ID | S1Q063 |
| Locus tag | LH372_RS09405 | Protein ID | WP_001259255.1 |
| Coordinates | 1954140..1954391 (+) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LH372_RS09380 (1949309) | 1949309..1950376 | - | 1068 | WP_000080093.1 | bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB | - |
| LH372_RS09385 (1950376) | 1950376..1951446 | - | 1071 | WP_000108939.1 | histidinol-phosphate transaminase | - |
| LH372_RS09390 (1951443) | 1951443..1952747 | - | 1305 | WP_001614652.1 | histidinol dehydrogenase | - |
| LH372_RS09395 (1952753) | 1952753..1953652 | - | 900 | WP_000131777.1 | ATP phosphoribosyltransferase | - |
| LH372_RS09400 (1953798) | 1953798..1953857 | - | 60 | WP_032145667.1 | his operon leader peptide | - |
| LH372_RS09405 (1954140) | 1954140..1954391 | + | 252 | WP_001259255.1 | YoeB-YefM toxin-antitoxin system antitoxin YefM | Antitoxin |
| LH372_RS09410 (1954388) | 1954388..1954642 | + | 255 | WP_000767829.1 | type II toxin-antitoxin system mRNA interferase toxin YoeB | Toxin |
| LH372_RS09415 (1954725) | 1954725..1955549 | + | 825 | WP_000754749.1 | SDR family oxidoreductase | - |
| LH372_RS09420 (1955595) | 1955595..1956524 | + | 930 | WP_000803351.1 | LysR substrate-binding domain-containing protein | - |
| LH372_RS09425 (1956739) | 1956739..1956801 | + | 63 | WP_010723108.1 | membrane protein YoeI | - |
| LH372_RS09430 (1956791) | 1956791..1958149 | + | 1359 | WP_000019197.1 | putrescine/proton symporter PlaP | - |
| LH372_RS09435 (1958328) | 1958328..1959386 | + | 1059 | WP_000492321.1 | thiosulfate utilization transporter TsuA/YeeE | - |
| LH372_RS09440 (1959400) | 1959400..1959627 | + | 228 | WP_000985273.1 | thiosulfate utilization sulfurtransferase TsuB/YeeD | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | galF / cpsACP / wzi / wza / gmd / wcaG / wcaJ / gnd / manC / manB / ugd | 1799111..2033559 | 234448 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 85 a.a. Molecular weight: 10183.58 Da Isoelectric Point: 8.0353
>T220362 WP_000767829.1 NZ_CP085074:1954388-1954642 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY
Download Length: 255 bp
>T220362 NZ_CP117010:707450-707746 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA
Antitoxin
Download Length: 84 a.a. Molecular weight: 9307.58 Da Isoelectric Point: 4.7954
>AT220362 WP_001259255.1 NZ_CP085074:1954140-1954391 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE
Download Length: 252 bp
>AT220362 NZ_CP117010:707748-708143 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|