Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 652003..652802 Replicon chromosome
Accession NZ_CP085074
Organism Escherichia coli strain P21_SE1_04.20

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag LH372_RS03140 Protein ID WP_000347275.1
Coordinates 652003..652467 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LH372_RS03145 Protein ID WP_001307405.1
Coordinates 652467..652802 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH372_RS03110 (647004) 647004..647438 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LH372_RS03115 (647456) 647456..648334 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LH372_RS03120 (648324) 648324..649103 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
LH372_RS03125 (649114) 649114..649587 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
LH372_RS03130 (649610) 649610..650890 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
LH372_RS03135 (651139) 651139..651948 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LH372_RS03140 (652003) 652003..652467 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LH372_RS03145 (652467) 652467..652802 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LH372_RS03150 (652951) 652951..654522 - 1572 WP_001273752.1 galactarate dehydratase -
LH372_RS03155 (654897) 654897..656231 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LH372_RS03160 (656247) 656247..657017 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T220356 WP_000347275.1 NZ_CP085074:c652467-652003 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T220356 NZ_CP117008:c4604696-4604346 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT220356 WP_001307405.1 NZ_CP085074:c652802-652467 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT220356 NZ_CP117008:c4604941-4604690 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References