Detailed information of TA system TA220351

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 43486..43750
• Replicon: plasmid unnamed1
• Accession: NZ_CP085071
• Organism: Escherichia coli strain P27_L1_05.20

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: LH371_RS25090
• Protein ID: WP_001387489.1
• Coordinates: 43486..43638 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	43690..43750 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH371_RS25050 (38578)	38578..39111	-	534	WP_001221666.1	lipocalin family protein	-
LH371_RS25055 (39205)	39205..40350	-	1146	WP_039023136.1	extended-spectrum class C beta-lactamase CMY-42	-
LH371_RS25065 (41555)	41555..41653	+	99	Protein_47	ethanolamine utilization protein EutE	-
LH371_RS25070 (41725)	41725..41934	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
LH371_RS25075 (42326)	42326..42502	+	177	WP_001054897.1	hypothetical protein	-
LH371_RS25080 (42567)	42567..42863	-	297	WP_011264046.1	DinQ-like type I toxin DqlB	-
LH371_RS25085 (43163)	43163..43414	+	252	WP_001291964.1	hypothetical protein	-
LH371_RS25090 (43486)	43486..43638	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (43690)	43690..43750	+	61	NuclAT_0	-	Antitoxin
- (43690)	43690..43750	+	61	NuclAT_0	-	Antitoxin
- (43690)	43690..43750	+	61	NuclAT_0	-	Antitoxin
- (43690)	43690..43750	+	61	NuclAT_0	-	Antitoxin
LH371_RS25095 (43959)	43959..44333	+	375	WP_223349261.1	hypothetical protein	-
LH371_RS25100 (44486)	44486..45643	+	1158	Protein_54	IS1380-like element ISEcp1 family transposase	-
LH371_RS25105 (45699)	45699..46396	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
LH371_RS25110 (46407)	46407..46715	-	309	WP_039023235.1	transcription termination/antitermination NusG family protein	-
LH371_RS25115 (47157)	47157..47444	-	288	WP_000074855.1	conjugal transfer protein TraA	-
LH371_RS25120 (47482)	47482..47640	-	159	Protein_58	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..48551	48551
	-	inside	IScluster/Tn	blaCMY-42	-	39205..45974	6769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T220351 WP_001387489.1 NZ_CP085071:c43638-43486 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T220351 NZ_CP117008:3457861-3458247 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT220351 NZ_CP085071:43690-43750 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin